Mouse Immunoglobulin G2a (IgG2a) Isotype Control

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Isotype
Host
Mouse
241
130
1
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Negative Control (NC), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Clone MOPC-173
Isotype IgG2a
Specificity This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
No Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Alternative Name IgG2a
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ,g/mL) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Research Area Secondary Antibodies
Application Notes The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µ,g per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Restrictions For Research Use only
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Gupta, Gylling, Alonso, Sugimori, Ianakiev, Xiong, Arnaout: "The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18." in: Blood, Vol. 109, Issue 8, pp. 3513-20, 2007 (PubMed).

Fougereau, Bourgois, de Preval, Rocca-Serra, Schiff: "The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications." in: Annales d'immunologie, Vol. 127, Issue 5, pp. 607-31, 1977 (PubMed).

Baumal, Scharff: "Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 116, Issue 1, pp. 65-74, 1976 (PubMed).