HEK-293 Cells IgG isotype control (AVI tag,Biotin)

Details for Product No. ABIN2870572, Supplier: Log in to see
Isotype
Reactivity
Mouse (Murine)
Host
HEK-293 Cells
219
210
208
172
162
130
114
94
93
92
91
76
59
52
44
42
27
25
24
23
22
11
8
6
3
2
1
Biological Activity
Active
Conjugate
AVI tag,Biotin
207
169
168
166
157
108
58
58
48
45
20
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Isotype Control (IsoC)
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Brand MABSol®,PrecisionAvi
Isotype IgG
Fragment Fc fragment
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
Molecular Weight 28.2 kDa
UniProt P01863
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for HEK-293 Cells IgG isotype control (AVI tag,Biotin) (ABIN2870572) Biotinylated Mouse IgG2a Fc, Tag Free on SDS-PAGE under reducing (R) condition. The g...
Binding Studies (Bind) image for HEK-293 Cells IgG isotype control (AVI tag,Biotin) (ABIN2870572) Immobilized Recombinant Protein A (Cat# RPA-S3149) at 5 μg/mL (100 μL/well) can bind ...