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Rabbit IgG isotype control (FITC)

IsoC, ELISA, WB, IP, DB, IEP, ID, BR IgG FITC
Catalog No. ABIN398848
  • Target See all IgG products
    IgG
    Host
    • 142
    • 140
    • 123
    • 89
    • 89
    • 57
    • 56
    • 47
    • 46
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 15
    • 9
    • 9
    • 4
    • 2
    Rabbit
    Conjugate
    FITC
    Application
    Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB), Immunoelectrophoresis (IEP), Immunodiffusion (ID), Blocking Reagent (BR)
    Characteristics
    Purified Rabbit IgG (Whole Molecule) Control [FITC] is highly purified from normal rabbit serum through Protein G chromatography. It is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
    Absorption Wavelength: 492 nm
    Emission Wavelength: 518 nm
    Fluorochrome/Protein Ratio: 4.8 moles FITC per mole of IgG
    Purification
    Protein G chromatography
    Isotype
    IgG
  • Application Notes
    Each Investigator should determine their own optimal working dilution for specific applications.
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    0.5 mg/mL
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Product is photosensitive and should be protected from light.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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