Human IgG isotype control (FITC)

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Isotype
Host
Human
217
207
204
168
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
Conjugate
FITC
206
169
167
166
153
108
58
58
48
44
19
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Isotype Control (IsoC)
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Isotype IgG
Fragment Whole molecule
Characteristics Purified Human IgG (Whole Molecule) Control [FITC] is highly purified from normal human serum through Protein G chromatography. It is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunoblotting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
Absorption Wavelength: 492 nm,
Emission Wavelength: 518 nm,
Fluorochrome/Protein Ratio: 4.1 moles FITC per mole of IgG
Purification Protein G chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Product cited in: Tomita, Fuchimoto, Mori, Kato, Uemura, Handa, Tazawa, Ohdan, Okamoto, Kuroda: "Production of anti-ABO blood group antibodies after minor ABO-incompatible bone marrow transplantation in NOD/SCID/gamma(c)(null) mice." in: Clinical transplantation, Vol. 27, Issue 6, pp. E702-8, 2013 (PubMed).

Lim, Kim, Aref, Kamm, Raghunath: "Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 5, Issue 12, pp. 1474-84, 2013 (PubMed).