Human IgG isotype control (FITC)

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Isotype
Host
Human
219
210
208
171
162
130
114
94
93
92
91
76
59
52
44
42
27
25
24
23
22
11
8
6
3
3
1
Conjugate
FITC
207
169
168
166
156
108
58
58
48
45
20
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Isotype Control (IsoC)
Options
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Isotype IgG
Fragment Whole molecule
Characteristics Purified Human IgG (Whole Molecule) Control [FITC] is highly purified from normal human serum through Protein G chromatography. It is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunoblotting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
Absorption Wavelength: 492 nm,
Emission Wavelength: 518 nm,
Fluorochrome/Protein Ratio: 4.1 moles FITC per mole of IgG
Purification Protein G chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Product cited in: Tomita, Fuchimoto, Mori, Kato, Uemura, Handa, Tazawa, Ohdan, Okamoto, Kuroda: "Production of anti-ABO blood group antibodies after minor ABO-incompatible bone marrow transplantation in NOD/SCID/gamma(c)(null) mice." in: Clinical transplantation, Vol. 27, Issue 6, pp. E702-8, 2013 (PubMed).

Lim, Kim, Aref, Kamm, Raghunath: "Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 5, Issue 12, pp. 1474-84, 2013 (PubMed).