Mouse IgG2b isotype control (APC)
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- Target See all IgG2b products
- IgG2b
- Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
- Conjugate
- APC
- Application
- Flow Cytometry (FACS), ELISA, FLISA, Fluorescence Microscopy (FM)
- Purification
- Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3 , IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition has been confirmed.
- Isotype
- IgG2b
- Labeling Ratio
- 1-2
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Mouse IgG2b isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin
Mouse IgG2b isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype control (PE)FACS Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (PerCP)FACS Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (APC)FACS Monoclonal MPC-11 IgG2b kappa APC
Mouse IgG2b isotype control (FITC)FACS, IsoC, IF IgG2b FITC
Mouse IgG2b isotype controlFACS, IsoC IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS, ELISA Monoclonal A-1 IgG2b FITC
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- Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2b kappa isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000-1:20,000
IF Microscopy Dilution: User Optimized
- Comment
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Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 3 and μ - IgM, light chain κ and λ.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
- Expiry Date
- 12 months
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- Target
- IgG2b
- Abstract
- IgG2b Products
- Synonyms
- IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
- Target Type
- Antibody
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