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Llama IgG isotype control (HRP)

ELISA, FACS, WB IgG HRP
Catalog No. ABIN5608820
  • Target See all IgG products
    IgG
    Reactivity
    Llama
    Host
    • 140
    • 136
    • 119
    • 89
    • 87
    • 57
    • 56
    • 47
    • 46
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 14
    • 9
    • 9
    • 4
    • 2
    Llama
    Conjugate
    HRP
    Application
    ELISA, Flow Cytometry (FACS), Western Blotting (WB)
    Purification
    Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
    Isotype
    IgG
  • Application Notes

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG2 HRP conjugated isotype control can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Comment

    Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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