Hamster IgG isotype control (HRP)

Details for Product No. ABIN930151, Supplier: Log in to see
Isotype
Host
Hamster
219
209
209
172
162
129
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Conjugate
HRP
207
167
165
164
157
108
58
58
48
45
25
13
12
12
11
9
9
6
6
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Isotype Control (IsoC)
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Isotype IgG
Fragment F(ab')2 fragement
Characteristics Purified Hamster IgG (Fab'2) HRP conjugate
Alternative Names: Hamster Immunoglobulin G (Fab'2) (HRP)
Physical state: Clear, colorless liquid
Purification Human IgG protein (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and dialysis.
Background Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen-binding site - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. Treatment with pepsin produces an F(ab)'2 fragment.
Synonyms: Hamster Immunoglobulin G (Fab'2) (HRP).
Research Area Immunology, Secondary Antibodies
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Restrictions For Research Use only
Format Lyophilized
Concentration Lot specific
Buffer Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamicin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage RT/-20 °C
Storage Comment Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Product cited in: Morris, Brown, Kong: "Naturally-existing CD4(+)CD25(+)Foxp3(+) regulatory T cells are required for tolerance to experimental autoimmune thyroiditis induced by either exogenous or endogenous autoantigen." in: Journal of autoimmunity, Vol. 33, Issue 1, pp. 68-76, 2009 (PubMed).