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Human IgG isotype control (Biotin)

IsoC, ELISA, WB IgG Native Biotin
Catalog No. ABIN964323
  • Target See all IgG products
    IgG
    Antibody Type
    Native
    Host
    • 142
    • 140
    • 124
    • 89
    • 88
    • 57
    • 56
    • 47
    • 46
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 15
    • 9
    • 9
    • 4
    • 2
    Human
    Conjugate
    Biotin
    Application
    Isotype Control (IsoC), ELISA, Western Blotting (WB)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Sterility
    Sterile filtered
    Isotype
    IgG
  • Application Notes
    Human IgG whole molecule Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Aliquot to Avoid repeated freezing and thawing.
    Storage
    4 °C
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin.

    Synonyms: Human immunoglobulin G, Vitamin H, coenzyme R
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