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Mouse IgG2b isotype control (FITC)

FACS, IsoC, ELISA, WB, FM Monoclonal M2B IgG2b FITC
Catalog No. ABIN964474
  • Target See all IgG2b products
    IgG2b
    Host
    • 130
    • 51
    • 2
    Mouse
    Clonality
    • 121
    • 2
    • 1
    Monoclonal
    Conjugate
    FITC
    Application
    Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Fluorescence Microscopy (FM)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Sterility
    Sterile filtered
    Clone
    • 27
    • 17
    • 15
    • 11
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    M2B
    Isotype
    IgG2b
  • Application Notes
    This product is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    4 °C
  • Target
    IgG2b
    Abstract
    IgG2b Products
    Synonyms
    IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
    Target Type
    Antibody
    Background
    Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

    Synonyms: FITC, Isotype Control
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