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anti-Mouse (Murine) 5HT1B Receptor antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended 5HT1B Receptor Antibody (supplied by: Log in to see )

Antigen
Serotonin Receptor 1B (HTR1B) Antibodies
  • 5-HT-1B
  • 5-HT-dro2B
  • 5-HT1-LIKE
  • 5-HT1B
  • 5-HT1BDro
  • 5-HT1DB
  • 5-HT1Db
  • 5-HT[1B]Dro
  • 5-HT[[1BDro]]
  • 5-HT[[1B]]
  • 5-HT[[1B]]Dro
  • 5-HT[[1B]]DRO
  • 5-HT[[dro2B]]
  • 5HT-dro2B
  • 5HT-dro[[2B]]
  • 5HT-R2B
  • 5HT1B
  • 5htdro2b
  • CG15113
  • CG30126
  • d5-HT1B
  • Dm5HTdro2B
  • Dmel\\CG15113
  • Dmel_CG30126
  • DRO2B
  • HTR1B
  • HTR1D2
  • HTR1DB
  • S12
Epitope
3rd Cytoplasmic Domain
10
9
7
2
2
2
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
25
8
8
1
1
1
1
Host
Rabbit
22
2
1
Clonality
Polyclonal
Conjugate
This 5HT1B Receptor antibody is un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
22
19
6
2
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Catalog No. ABIN271207
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Top referenced anti-5HT1B Receptor antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-5HT1B Receptor Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Pig (Porcine) Cow (Bovine) Bat Guinea Pig Hamster Rabbit
Western Blotting (WB) 23 Antibodies 7 Antibodies 7 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies

General

Antigen Serotonin Receptor 1B (HTR1B) Antibodies
Epitope 3rd Cytoplasmic Domain
(10), (9), (7), (2), (2), (2), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(25), (8), (8), (1), (1), (1), (1)
Host Rabbit
(22), (2), (1)
Clonality
Polyclonal
Conjugate This 5HT1B Receptor antibody is un-conjugated
(1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(22), (19), (6), (2)
Pubmed 1 reference available
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Product Details anti-5HT1B Receptor Antibody

Target Details 5HT1B Receptor Application Details Handling References for anti-5HT1B Receptor Antibody (ABIN271207) Images
Specificity Human 5HT1B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Cross-Reactivity (Details) There is 100 % rat,identity,100 % rat,homology. There is 100 % mouse identity,100 % mouse homology.
Purification Immunogen affinity purified
Immunogen A 16 synthetic amino acid peptide within the 3rd cytoplasmic domain of human 5HT1B Receptor.

Target Details 5HT1B Receptor

Product Details anti-5HT1B Receptor Antibody Application Details Handling References for anti-5HT1B Receptor Antibody (ABIN271207) Images back to top
Antigen
Alternative Name 5-HT1B (HTR1B Antibody Abstract)
Background Gene Symbol: HTR1B
Pathways JAK-STAT Signaling

Application Details

Product Details anti-5HT1B Receptor Antibody Target Details 5HT1B Receptor Handling References for anti-5HT1B Receptor Antibody (ABIN271207) Images back to top
Application Notes Immunohistochemistry-Paraffin 7 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for 5HT1B Receptor Antibody (NLS598)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

Product Details anti-5HT1B Receptor Antibody Target Details 5HT1B Receptor Application Details References for anti-5HT1B Receptor Antibody (ABIN271207) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS.
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-5HT1B Receptor Antibody (ABIN271207)

Product Details anti-5HT1B Receptor Antibody Target Details 5HT1B Receptor Application Details Handling Images back to top
Product cited in:

Guilluy, Rolli-Derkinderen, Tharaux, Melino, Pacaud, Loirand: "Transglutaminase-dependent RhoA activation and depletion by serotonin in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 282, Issue 5, pp. 2918-28, 2007