anti-Human AGT antibody for Flow Cytometry

Recommended AGT Antibody (supplied by: Log in to see )

Antigen
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
  • wu:fb62f06
  • wu:fj87b02
  • zgc:111892
  • AGT
  • angt
  • SPT
  • Spat
  • ANHU
  • SERPINA8
  • AI265500
  • AngI
  • AngII
  • Aogen
  • Serpina8
  • ANRT
  • Ang
  • PAT
  • ANGT
  • AI267024
  • Agat
  • angiotensinogen
  • Angiotensinogen
  • alanine-glyoxylate aminotransferase
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • angiotensinogen-like
  • O-6-methylguanine-DNA methyltransferase
  • agt
  • AGT
  • angt
  • Agxt
  • Agt
  • LOC100157073
  • LOC100065526
  • Mgmt
Epitope
AA 1-485
40
15
13
13
8
8
7
5
5
5
5
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
205
58
52
8
2
2
2
2
2
2
1
Host
Mouse
154
85
2
1
1
Clonality
Monoclonal
Conjugate
This AGT antibody is un-conjugated
15
11
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
149
108
69
47
22
20
14
14
7
7
4
3
3
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1997921
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.634827 ABIN1997928 FACS FITC Rabbit IgG AA 1-485 Log in to see 001
14.634827 ABIN1997930 FACS PE Rabbit IgG AA 1-485 Log in to see 001
14.634827 ABIN1997924 FACS PE Mouse IgG2a AA 1-485 Log in to see 08

General

Antigen Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
Epitope AA 1-485
(40), (15), (13), (13), (8), (8), (7), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(205), (58), (52), (8), (2), (2), (2), (2), (2), (2), (1)
Host Mouse
(154), (85), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AGT antibody is un-conjugated
(15), (11), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(149), (108), (69), (47), (22), (20), (14), (14), (7), (7), (4), (3), (3), (2), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-AGT Antibody

Target Details AGT Application Details Handling Images
Specificity Human SerpinA8 / Angiotensinogen / AGT
No cross-reactivity in ELISA
Human SerpinA1
Human SerpinA3
Human SerpinA5
Human SerpinC1
Human SerpinD1
Human SerpinF2
Human SerpinG1
Human SerpinI1
No Cross-Reactivity Human
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinA8 / Angiotensinogen / AGT (rh SerpinA8 / Angiotensinogen / AGT, ABIN2003702 NP_000020.1, Met 1-Ala 485,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human SerpinA8 / Angiotensinogen / AGT protein (ABIN2003702)
Clone 08
Isotype IgG2a

Target Details AGT

Product Details anti-AGT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AGT (AGT Antibody Abstract)
Background Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is anα-2-globulinthat is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).
NCBI Accession NP_000020.1
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-AGT Antibody Target Details AGT Handling Images back to top
Application Notes ICC/IF: 10-50 μg/mL
FCM: 0.5-2 μg/Test
Restrictions For Research Use only

Handling

Product Details anti-AGT Antibody Target Details AGT Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-AGT Antibody Target Details AGT Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-485) antibody (ABIN1997921) Human AGT expression in HepG2 cells. The cells were treated according to manufacturer...