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anti-Human AGT antibody for Flow Cytometry

Recommended AGT Antibody (supplied by: Log in to see )

Antigen
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
  • Agat
  • AGT
  • AI265500
  • AI267024
  • Ang
  • AngI
  • AngII
  • angt
  • ANGT
  • ANHU
  • ANRT
  • Aogen
  • PAT
  • Serpina8
  • SERPINA8
  • Spat
  • SPT
  • wu:fb62f06
  • wu:fj87b02
  • zgc:111892
Epitope
AA 1-485
28
12
12
10
8
7
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
166
52
48
7
2
2
1
1
1
1
Host
Mouse
133
64
2
1
1
Clonality
Monoclonal
Conjugate
This AGT antibody is un-conjugated
14
10
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
113
88
44
26
12
12
10
8
6
3
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN1997921
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2982299 ABIN1997924 FACS PE Mouse IgG2a AA 1-485 Log in to see 8
0.2982299 ABIN1997928 FACS FITC Rabbit IgG AA 1-485 Log in to see 1
0.2982299 ABIN1997930 FACS PE Rabbit IgG AA 1-485 Log in to see

Similar anti-AGT Antibodies

Application / Reactivity Human
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 95 Antibodies

General

Antigen Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
Epitope AA 1-485
(28), (12), (12), (10), (8), (7), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(166), (52), (48), (7), (2), (2), (1), (1), (1), (1)
Host Mouse
(133), (64), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AGT antibody is un-conjugated
(14), (10), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(113), (88), (44), (26), (12), (12), (10), (8), (6), (3), (3), (3), (2), (1), (1), (1), (1)
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Product Details anti-AGT Antibody

Target Details AGT Application Details Handling Images
Specificity Human SerpinA8 / Angiotensinogen / AGT
Cross-Reactivity (Details) No cross-reactivity in ELISA with Human SerpinA1 Human SerpinA3 Human SerpinA5 Human SerpinC1 Human SerpinD1 Human SerpinF2 Human SerpinG1 Human SerpinI1
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinA8 / Angiotensinogen / AGT (rh SerpinA8 / Angiotensinogen / AGT, NP_000020.1, Met 1-Ala 485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Sterility 0.2 μm filtered
Immunogen Recombinant Human SerpinA8 / Angiotensinogen / AGT protein
Clone 8
Isotype IgG2a

Target Details AGT

Product Details anti-AGT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AGT (AGT Antibody Abstract)
Background Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is anα-2-globulinthat is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).
NCBI Accession NP_000020.1
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-AGT Antibody Target Details AGT Handling Images back to top
Application Notes Flow Cytometry - Human AGT expression in HepG2 cells. The cells were treated according to manufacturer's manual , and stained with Purified Mouse anti-AGT (10994-MM08, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.
Restrictions For Research Use only

Handling

Product Details anti-AGT Antibody Target Details AGT Application Details Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

Images

Product Details anti-AGT Antibody Target Details AGT Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-AGT antibody (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8)) (AA 1-485) (ABIN1997921) Human AGT expression in HepG2 cells. The cells were treated according to manufacturer...