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anti-Human AGT antibody for Immunofluorescence

Recommended AGT Antibody (supplied by: Log in to see )

Antigen
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
  • Agat
  • AGT
  • AI265500
  • AI267024
  • Ang
  • AngI
  • AngII
  • angt
  • ANGT
  • ANHU
  • ANRT
  • Aogen
  • PAT
  • Serpina8
  • SERPINA8
  • Spat
  • SPT
  • wu:fb62f06
  • wu:fj87b02
  • zgc:111892
Epitope
AA 1-485
43
12
12
12
10
8
7
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
207
52
48
7
2
2
1
1
1
1
Host
Rabbit
152
87
2
1
1
Clonality
Monoclonal
Conjugate
This AGT antibody is un-conjugated
16
12
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
130
111
60
41
21
19
13
12
6
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1997925
$ 273.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.27038813 ABIN2855709 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.27038813 ABIN2156981 IHC (fp) IF WB Rabbit IgG Log in to see Polyclonal
0.0011012675 ABIN447446 ICC IF IHC (p) IHC WB Rabbit N-Term, AA 1-50 Log in to see Polyclonal 6
0.0011012675 ABIN1885927 IF IHC WB Rabbit AA 1-446 Log in to see Polyclonal
0.0011012675 ABIN2799931 IF (cc) IHC (f) IF IHC (p) IHC WB Rabbit IgG AA 1-476 Log in to see Polyclonal
0.0011012675 ABIN4352879 ICC IF IHC IHC (p) WB Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352883 ICC IF IHC IHC (p) WB Biotin Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352885 ICC IF IHC IHC (p) WB DyLight 488 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352884 ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352887 ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352889 ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352890 ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352893 ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352892 ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN4352891 ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 1-50, N-Term Log in to see Polyclonal
0.0011012675 ABIN796710 IF IHC Rabbit IgG Log in to see Polyclonal
0.0011012675 ABIN2200818 IF IHC Rabbit IgG Log in to see Polyclonal
0.0011012675 ABIN4352886 ICC IF IHC IHC (p) WB HRP Rabbit AA 1-50, N-Term Log in to see Polyclonal

Top referenced anti-AGT antibody for Immunofluorescence

  • Human Polyclonal AGT Primary Antibody for ICC, IF - ABIN447446 (6 Publications): Dimitrijevic, Rissler, Luts, Edvinsson: Reduced expression of angiotensin II and angiotensin receptor type 1 and type 2 in resistance arteries from nasal lesions in granulomatosis with polyangiitis (Wegener's granulomatosis). in Scandinavian journal of rheumatology 2011 (PubMed)

Similar anti-AGT Antibodies

Application / Reactivity Human
ELISA 96 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 52 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 112 Antibodies

General

Antigen Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
Epitope AA 1-485
(43), (12), (12), (12), (10), (8), (7), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(207), (52), (48), (7), (2), (2), (1), (1), (1), (1)
Host Rabbit
(152), (87), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AGT antibody is un-conjugated
(16), (12), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(130), (111), (60), (41), (21), (19), (13), (12), (6), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-AGT Antibody

Target Details AGT Application Details Handling Images
Specificity Human SerpinA8 / Angiotensinogen
Cross-Reactivity (Details) No cross-reactivity in ELISA with Human SerpinA1 / A1AT Human SerpinA3 / SERPINA3 / AACT Human SerpinA5 / Protein C inhibitor Human SerpinD1 / HC2 Human SerpinF2 / SERPINF2 Human SerpinG1 / C1IN Human SerpinI1 / Protease inhibitor 12 Human SerpinC1 / SERPINC1 / Antithrombin-III
Sensitivity 0.0195 ng/well
Characteristics This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human SerpinA8 / angiotensinogen ( rh SerpinA8 , NP_000020.1 , Met 1 - Ala 485 ).
Sterility 0.2 μm filtered
Immunogen Recombinant human SerpinA8 protein
Clone 1
Isotype IgG

Target Details AGT

Product Details anti-AGT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AGT (AGT Antibody Abstract)
Background Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).
NCBI Accession NP_000020.1
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-AGT Antibody Target Details AGT Handling Images back to top
Application Notes Western blot - WB: 2-5 μg/mL Direct ELISA - This antibody can be used at 0.1 -0.2 μg/mL with the appropriate secondary reagents to detect human AGT. The detection limit for human AGT is 0.0195 ng/well Immunochemistry - ICC/IF: 10-25 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-AGT Antibody Target Details AGT Application Details Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

Images

Product Details anti-AGT Antibody Target Details AGT Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-AGT antibody (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8)) (AA 1-485) (ABIN1997925) Immunofluorescence staining of Human AGT in Hela or SKBR3 cells. Cells were fixed wit...
Western Blotting (WB) image for anti-AGT antibody (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8)) (AA 1-485) (ABIN1997925) anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-485) antibody (Image 2)