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anti-Mouse (Murine) AGT antibody for Immunocytochemistry

Recommended AGT Antibody (supplied by: Log in to see )

Antigen
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
  • Agat
  • AGT
  • AI265500
  • AI267024
  • Ang
  • AngI
  • AngII
  • angt
  • ANGT
  • ANHU
  • ANRT
  • Aogen
  • PAT
  • Serpina8
  • SERPINA8
  • Spat
  • SPT
  • wu:fb62f06
  • wu:fj87b02
  • zgc:111892
Epitope
N-Term, AA 1-50
28
13
12
10
8
7
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
167
52
48
7
2
2
1
1
1
1
Host
Rabbit
133
65
2
1
1
Clonality
Polyclonal
Conjugate
This AGT antibody is un-conjugated
14
10
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
113
88
44
26
12
12
10
8
6
4
3
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN447446
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161058 ABIN2855709 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0010161058 ABIN1173617 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 23-477 Log in to see Polyclonal

Similar anti-AGT Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 16 Antibodies 19 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 3 Antibodies
Immunofluorescence (IF) 3 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 18 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 6 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 23 Antibodies

General

Antigen Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
Epitope N-Term, AA 1-50
(28), (13), (12), (10), (8), (7), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(167), (52), (48), (7), (2), (2), (1), (1), (1), (1)
Host Rabbit
(133), (65), (2), (1), (1)
Clonality
Polyclonal
Conjugate This AGT antibody is un-conjugated
(14), (10), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(113), (88), (44), (26), (12), (12), (10), (8), (6), (4), (3), (3), (2), (1), (1), (1), (1)
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Product Details anti-AGT Antibody

Target Details AGT Application Details Handling Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an N-terminal portion of the human Angiotensinogen protein (between residues 1-50) [UniProt P01019]

Target Details AGT

Product Details anti-AGT Antibody Application Details Handling Images back to top
Antigen
Alternative Name Serpin A8/Angiotensinogen (AGT Antibody Abstract)
Background Gene Symbol: AGT
Molecular Weight Theoretical MW: 53 kDa
UniProt P01019
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-AGT Antibody Target Details AGT Handling Images back to top
Application Notes Western Blot 1:1000, Immunohistochemistry 1:40-1:100, Immunocytochemistry/Immunofluorescence 1:40-1:100, Immunohistochemistry-Paraffin 1:50-1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocol specific for Angiotensin antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol specific for Angiotensin antibody Immunohistochemistry-Paraffin Embedded Sections ProtocolAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-AGT Antibody Target Details AGT Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS with 30 % glycerol.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-AGT Antibody Target Details AGT Application Details Handling back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-AGT antibody (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8)) (N-Term) (ABIN447446) anti-Angiotensinogen (Serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-50), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-AGT antibody (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8)) (N-Term) (ABIN447446) anti-Angiotensinogen (Serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-50), (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-AGT antibody (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8)) (N-Term) (ABIN447446) anti-Angiotensinogen (Serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-50), (N-Term) antibody (Image 3)