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|Antigen||Cardiotrophin 1 (CTF1) Antibodies|
|Conjugate||This Cardiotrophin 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-Cardiotrophin 1 AntibodyTarget Details Cardiotrophin 1 Application Details Handling Images
E.coli derived recombinant Human Cardiotrophin-1.
Type of Immunogen: Cell extract
Target Details Cardiotrophin 1Product Details anti-Cardiotrophin 1 Antibody Application Details Handling Images back to top
|Alternative Name||CFT1 / CT-1 / Cardiotrophin-1 (CTF1 Antibody Abstract)|
Name/Gene ID: CTF1
Synonyms: CTF1, Cardiotrophin 1, Cardiotrophin-1, CT1, Cardiophin 1, CT-1
Application DetailsProduct Details anti-Cardiotrophin 1 Antibody Target Details Cardiotrophin 1 Handling Images back to top
Approved: ELISA, IHC-P (0.25 μg/mL), Neut (0.25 - 0.4 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCardiotrophin-1 (15 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect hCT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CT-1 (LS-C104694) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCT-1. Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3 ng/lane, under reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cardiotrophin 1 Antibody Target Details Cardiotrophin 1 Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-Cardiotrophin 1 Antibody Target Details Cardiotrophin 1 Application Details Handling back to top