Human Cardiotrophin 1 ELISA Kit

Recommended Cardiotrophin 1 Product (supplied by: Log in to see )

Antigen
Cardiotrophin 1 (CTF1) ELISA Kits
  • CTF1
  • ct1
  • ct-1
  • ctf2
  • CT-1
  • CT1
  • cardiotrophin 1
  • cardiotrophin-1-like
  • CTF1
  • ctf1
  • Ctf1
  • LOC100736818
Epitope
AA 2-201
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
14
11
3
2
2
2
2
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
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Catalog No. ABIN411375
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.537022 ABIN365075 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
9.537022 ABIN625282 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 20-12000 pg/mL 20 pg/mL Log in to see
9.537022 ABIN1113967 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
9.537022 ABIN2708086 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
9.537022 ABIN2113598 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
1 ABIN578983 Sandwich ELISA Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN509900 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN1569223 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN5520303 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1112578 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN1502931 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 25-500 pg/mL 25 pg/mL Log in to see
1 ABIN2042369 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2683952 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN1846868 Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2542475 Serum, Plasma, Biological Fluids 3-200 pg/mL 3 pg/mL Log in to see
1 ABIN4967760 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2542478 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
1 ABIN2115902 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2946100 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN5073628 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen Cardiotrophin 1 (CTF1) ELISA Kits
Epitope AA 2-201
Reactivity Human
Kits with alternative reactivity to:
(21), (14), (11), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details Cardiotrophin 1 ELISA Kit

Target details Application Details Handling ProductDetails: References for Cardiotrophin 1 Kit (ABIN411375) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cardiotrophin-1
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: E.coli
Immunogen sequence: S2-A201
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S2-A201

Target details

Product Details Cardiotrophin 1 ELISA Kit Application Details Handling ProductDetails: References for Cardiotrophin 1 Kit (ABIN411375) Images back to top
Antigen
Alternative Name CTF1 (CTF1 ELISA Kit Abstract)
Background

Protein Function: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

Synonyms: Cardiotrophin-1,CT-1,CTF1,

Full Gene Name: Cardiotrophin-1

Cellular Localisation: Secreted.
Gene ID 1489
UniProt Q16619
Pathways JAK-STAT Signaling

Application Details

Product Details Cardiotrophin 1 ELISA Kit Target details Handling ProductDetails: References for Cardiotrophin 1 Kit (ABIN411375) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.

Plate Pre-coated
Protocol human CT-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CT-1 has been precoated onto 96-well plates. Standards(E.coli, S2-A201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CT-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CT-1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 253, Standard deviation: 11.38, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 759, Standard deviation: 35.67, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 2042, Standard deviation: 98.09, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 264, Standard deviation: 12.14, CV(%): 4.6
  • Sample 2: n=24, Mean(pg/ml): 788, Standard deviation: 37.82, CV(%): 4.8
  • Sample 3: n=24, Mean(pg/ml): 1946, Standard deviation: 97.30, CV(%): 5.0
Restrictions For Research Use only

Handling

Product Details Cardiotrophin 1 ELISA Kit Target details Application Details ProductDetails: References for Cardiotrophin 1 Kit (ABIN411375) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for Cardiotrophin 1 Kit (ABIN411375)

Product Details Cardiotrophin 1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Pennica, Swanson, Shaw, Kuang, Gray, Beatty, Wood: "Human cardiotrophin-1: protein and gene structure, biological and binding activities, and chromosomal localization." in: Cytokine, Vol. 8, Issue 3, pp. 183-9, 1997

Images

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Supplier Images
ELISA image for Cardiotrophin 1 ELISA Kit (CTF1) (ABIN411375) Human Cardiotrophin-1 PicoKine ELISA Kit standard curve