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Human Cardiotrophin 1 ELISA Kit

Recommended Cardiotrophin 1 Product (supplied by: Log in to see )

Antigen
Cardiotrophin 1 (CTF1) ELISA Kits
  • CT-1
  • ct-1
  • CT1
  • ct1
  • CTF1
  • ctf2
Epitope
AA 2-201
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
9
8
3
3
3
3
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
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Catalog No. ABIN411375
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30838606 ABIN1113967 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.30838606 ABIN2708086 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.30838606 ABIN2113598 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012131304 ABIN365075 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012131304 ABIN509900 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012131304 ABIN578983 Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012131304 ABIN625282 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012131304 ABIN1112578 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012131304 ABIN2042369 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012131304 ABIN2683952 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012131304 ABIN1846868 Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012131304 ABIN2542475 Serum, Plasma, Biological Fluids 3-200 pg/mL 3 pg/mL Log in to see
0.0012131304 ABIN2542478 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012131304 ABIN2070858 Sandwich ELISA Log in to see
0.0012131304 ABIN2115902 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012131304 ABIN2946100 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012131304 ABIN1719993 Log in to see

Top referenced Cardiotrophin 1 ELISA Kit for ELISA

Similar Cardiotrophin 1 ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Cardiotrophin 1 (CTF1) ELISA Kits
Epitope AA 2-201
Reactivity Human
Kits with alternative reactivity to:
(17), (9), (8), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details Cardiotrophin 1 ELISA Kit

Target details Application Details Handling References for Cardiotrophin 1 Kit (ABIN411375) Images
Purpose For quantitative detection of human CT-1 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human CT-1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human Cardiotrophin-1 antibod - 1
  • Lyophilized recombinant human Cardiotrophin-1 standar - 10ng/tubex2
  • Biotinylated anti- human Cardiotrophin-1 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S2-A201

Target details

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Antigen
Alternative Name CTF1 (CTF1 ELISA Kit Abstract)
Background Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.
Gene ID 1489
UniProt Q16619
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human CT-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CT-1 has been precoated onto 96-well plates. Standards(E.coli, S2-A201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CT-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CT-1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 253, Standard deviation: 11.38, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 759, Standard deviation: 35.67, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 2042, Standard deviation: 98.09, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 264, Standard deviation: 12.14, CV(%): 4.6
  • Sample 2: n=24, Mean(pg/ml): 788, Standard deviation: 37.82, CV(%): 4.8
  • Sample 3: n=24, Mean(pg/ml): 1946, Standard deviation: 97.30, CV(%): 5.0
Restrictions For Research Use only

Handling

Product Details Cardiotrophin 1 ELISA Kit Target details Application Details References for Cardiotrophin 1 Kit (ABIN411375) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Cardiotrophin 1 Kit (ABIN411375)

Product Details Cardiotrophin 1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Pennica, Swanson, Shaw, Kuang, Gray, Beatty, Wood: "Human cardiotrophin-1: protein and gene structure, biological and binding activities, and chromosomal localization." in: Cytokine, Vol. 8, Issue 3, pp. 183-9, 1997

Images

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Supplier Images
ELISA image for Cardiotrophin 1 ELISA Kit (CTF1) (ABIN411375) Human Cardiotrophin-1 PicoKine ELISA Kit standard curve