anti-Cow (Bovine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Cow (Bovine), Sheep (Ovine)
591
472
166
21
18
15
9
8
6
6
4
4
3
3
2
1
Host
Mouse
589
398
151
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to FITC
131
117
95
56
30
29
28
24
23
22
18
16
14
12
12
12
12
12
10
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
931
187
168
156
125
90
85
83
61
29
21
21
19
17
13
12
9
7
6
6
6
5
3
2
1
1
1
1
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Catalog No. ABIN181070
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2479442 IHC (fro) FACS IP Mouse IgG1 Log in to see IL-A111 4
1 ABIN2479443 FACS FITC Mouse IgG1 Log in to see IL-A111 4
1 ABIN596065 FACS PE Mouse IgG1 Log in to see
1 ABIN316523 FACS PE Mouse IgG1 Log in to see IL-A111
1 ABIN2479444 FACS PE Mouse IgG1 Log in to see IL-A111 4
1 ABIN596064 FACS FITC Mouse IgG1 Log in to see
1 ABIN601651 IHC (fro) FACS IP Mouse IgG1 Log in to see
1 ABIN5625390 FACS Func IHC Mouse IgG1 Log in to see Bu-CACT116A
1 ABIN5625392 FACS Func IHC Mouse IgG2a Log in to see CACT108A
1 ABIN5625399 FACS Func IHC Mouse IgG3 Log in to see LCTB2A
1 ABIN5625396 FACS Func IHC Mouse IgG1 Log in to see GB112A
1 ABIN5625393 FACS Func IHC Mouse IgG1 Log in to see CACT116A
1 ABIN576883 FACS IHC (fro) IP PE Mouse IgG1 Log in to see IL-A111
1 ABIN2288560 FACS PE Mouse IgG1 Log in to see 10B1132
1 ABIN2288558 FACS FITC Mouse IgG1 Log in to see 10B1132
1 ABIN2288556 FACS IP IHC Mouse IgG1 Log in to see 10B1132
1 ABIN206834 FACS IHC (fro) IP FITC Mouse IgG1 Log in to see
1 ABIN641705 FACS PE Mouse Log in to see
1 ABIN641704 FACS FITC Mouse Log in to see

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Cow (Bovine), Sheep (Ovine)
(591), (472), (166), (21), (18), (15), (9), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(589), (398), (151), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to FITC
(131), (117), (95), (56), (30), (29), (28), (24), (23), (22), (18), (16), (14), (12), (12), (12), (12), (12), (10), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(931), (187), (168), (156), (125), (90), (85), (83), (61), (29), (21), (21), (19), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN181070) Images
Specificity This antibody recognises the bovine CD25 cell surface antigen, a 55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain, which is expressed by activated T cells. Cross reacts with sheep. Other species not tested.
Characteristics Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen
Purification Affinity chromatography on Protein G
Clone IL-A111
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN181070) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 281861
UniProt P12342
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN181070) Images back to top
Application Notes Flow cytometry (Neat - 1/5): use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN181070) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD25 antibody (ABIN181070)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Howard, Sopp, Brownlie, Kwong, Parsons, Taylor: "Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 11, pp. 5372-82, 1998

Howard, Naessens: "Summary of workshop findings for cattle (tables 1 and 2)." in: Veterinary immunology and immunopathology, Vol. 39, Issue 1-3, pp. 25-47, 1994