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|Application / Reactivity||Cow (Bovine)||Sheep (Ovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Western Blotting (WB)||2 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies||5 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies||5 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Flow Cytometry (FACS)||17 Antibodies||15 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Cow (Bovine), Sheep (Ovine) Alternatives|
|Conjugate||This CD25 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
|2 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN181070) Images
|Specificity||This antibody recognises the bovine CD25 cell surface antigen, a 55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain, which is expressed by activated T cells. Cross reacts with sheep. Other species not tested.|
|Characteristics||Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen|
|Purification||Affinity chromatography on Protein G|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN181070) Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN181070) Images back to top
Flow cytometry (Neat - 1/5): use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN181070) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD25 Antibody (ABIN181070)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Howard, Sopp, Brownlie, Kwong, Parsons, Taylor: "Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 11, pp. 5372-82, 1998
Howard, Naessens: "Summary of workshop findings for cattle (tables 1 and 2)." in: Veterinary immunology and immunopathology, Vol. 39, Issue 1-3, pp. 25-47, 1994