anti-Cow (Bovine) CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
AA 22-243
33
33
23
22
20
19
15
15
13
13
7
6
6
6
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
604
498
175
21
19
15
10
8
6
6
4
4
3
3
2
1
Host
Rabbit
610
424
151
8
6
3
2
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
137
119
96
62
36
31
31
26
23
22
21
16
14
12
12
12
12
12
12
11
10
10
10
10
9
8
6
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
980
186
170
155
126
90
85
83
61
28
26
21
20
17
13
12
9
7
6
6
6
5
3
2
1
1
1
1
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Catalog No. ABIN1862763
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2935451 ELISA IF/ICC IHC WB Rabbit AA 22-243 Log in to see Polyclonal

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 22-243
(33), (33), (23), (22), (20), (19), (15), (15), (13), (13), (7), (6), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(604), (498), (175), (21), (19), (15), (10), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Rabbit
(610), (424), (151), (8), (6), (3), (2), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(137), (119), (96), (62), (36), (31), (31), (26), (23), (22), (21), (16), (14), (12), (12), (12), (12), (12), (12), (11), (10), (10), (10), (10), (9), (8), (6), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(980), (186), (170), (155), (126), (90), (85), (83), (61), (28), (26), (21), (20), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against IL2Ra. It has been selected for its ability to recognize IL2Ra in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen IL2Ra (Glu22-Gln243)
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 2 Receptor Alpha (IL2RA Antibody Abstract)
Background Alternative Names: CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-243) antibody (ABIN1862763) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-243) antibody