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anti-Human CD25 antibody for Biochemical Assay

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
568
457
169
19
10
10
8
5
4
3
3
2
1
1
Host
Mouse
601
406
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to PE-Cy5
130
124
101
61
30
28
25
25
22
18
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Biochemical Assay (BCA)
937
145
141
129
127
121
80
72
48
21
18
18
17
11
10
9
6
6
4
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2658947
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008857831 ABIN2658550 BCA APC Mouse IgG1 kappa Log in to see BC96 5
0.0008857831 ABIN2658948 BCA PE-Cy5 Mouse IgG1 kappa Log in to see BC96 5
0.0008857831 ABIN2658551 BCA APC Mouse IgG1 kappa Log in to see BC96 5

Top referenced anti-CD25 antibody for Biochemical Assay

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 61 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 420 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 68 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(568), (457), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(601), (406), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to PE-Cy5
(130), (124), (101), (61), (30), (28), (25), (25), (22), (18), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Biochemical Assay (BCA)
(937), (145), (141), (129), (127), (121), (80), (72), (48), (21), (18), (18), (17), (11), (10), (9), (6), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2658947) Images
Cross-Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Pigtailed Macaque
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Clone BC96
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2658947) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2658947) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2658947) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 Antibody (ABIN2658947)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Sutavani, Bradley, Ramage, Jackson, Durrant, Spendlove: "CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 12, pp. 5895-903, 2013

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Ernst, Lee, Nakanishi, Karimbux, Rezende, Stashenko, Seki, Taubman, Kawai: "Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease." in: Clinical and experimental immunology, Vol. 148, Issue 2, pp. 271-80, 2007

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2658947) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (PE-Cy5) (ABIN2658947) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PE-Cy5)