anti-Human CD25 antibody for Biochemical Assay

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human
603
498
175
22
19
15
10
8
6
6
4
4
3
3
2
1
Host
Mouse
609
424
152
8
6
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to APC
137
119
96
61
36
31
31
26
23
22
21
16
14
12
12
12
12
12
12
11
10
10
10
10
9
8
6
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Biochemical Assay (BCA)
980
187
171
156
126
90
85
83
61
29
26
21
20
17
13
12
9
7
6
6
6
4
3
2
1
1
1
1
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Catalog No. ABIN2658550
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658947 BCA PE-Cy5 Mouse IgG1 kappa Log in to see BC96 5
1 ABIN2658551 BCA APC Mouse IgG1 kappa Log in to see BC96 5
1 ABIN2658948 BCA PE-Cy5 Mouse IgG1 kappa Log in to see BC96 5

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(603), (498), (175), (22), (19), (15), (10), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(609), (424), (152), (8), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to APC
(137), (119), (96), (61), (36), (31), (31), (26), (23), (22), (21), (16), (14), (12), (12), (12), (12), (12), (12), (11), (10), (10), (10), (10), (9), (8), (6), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Biochemical Assay (BCA)
(980), (187), (171), (156), (126), (90), (85), (83), (61), (29), (26), (21), (20), (17), (13), (12), (9), (7), (6), (6), (6), (4), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2658550) Images
Cross-Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Pigtailed Macaque
Purification The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone BC96
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN2658550) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN2658550) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN2658550) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 antibody (ABIN2658550)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Trabanelli, Očadlíková, Ciciarello, Salvestrini, Lecciso, Jandus, Metz, Evangelisti, Laury-Kleintop, Romero, Prendergast, Curti, Lemoli: "The SOCS3-independent expression of IDO2 supports the homeostatic generation of T regulatory cells by human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 3, pp. 1231-40, 2014

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Ernst, Lee, Nakanishi, Karimbux, Rezende, Stashenko, Seki, Taubman, Kawai: "Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease." in: Clinical and experimental immunology, Vol. 148, Issue 2, pp. 271-80, 2007

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2658550) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (APC) (ABIN2658550) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (APC)