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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||4 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Peptide (BP)||4 Antibodies|
|Cytometry by Time of Flight (CyTOF)||8 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||407 Antibodies|
|Functional Studies (Func)||13 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||63 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||80 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||18 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling Images
|Specificity||This antibody detects Human CD25.|
|Characteristics||Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Highly pure (>98%) recombinant Human soluble IL-2 Receptor alpha|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman sIL-2RA (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required,in the presence of 0.5 ng/mL recombinant hIL-2. ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human sIL-2RA when used inconjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2RA by Western Blot analysis this antibody can be usedat a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human sIL-2RA is 2.0-4.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 1.0 mg/mL.|
|Buffer||PBS without preservatives|
|Storage||4 °C/-20 °C|
The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2 - 8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|