anti-Human CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human
576
453
152
17
15
9
8
6
5
4
3
2
1
1
Host
Mouse
564
393
149
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Biotin
131
116
92
56
29
28
28
23
23
22
17
16
12
11
11
11
11
11
10
9
8
8
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
915
156
155
153
109
85
85
72
48
21
19
17
16
14
13
12
9
7
6
6
6
5
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1106144
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.342041 ABIN1106146 FACS FITC Mouse IgG1 Log in to see B-F2 1
13.342041 ABIN1106135 Func IHC (fro) FACS Mouse IgG1 Log in to see B-F2 1
13.342041 ABIN1106134 Func FACS Mouse IgG1 Log in to see B-B10 18
13.342041 ABIN1106136 FACS Mouse IgG1 Log in to see B-G3 1
13.342041 ABIN264241 FACS PE Mouse IgG1 Log in to see 1TYV 4
13.342041 ABIN4896454 FACS PE Mouse IgG2a AA 22-213 Log in to see 24212 5
13.342041 ABIN4896455 FACS PE Mouse IgG2a AA 22-213 Log in to see 24212 5
13.342041 ABIN2660756 FACS Biotin Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2662232 FACS Pacific Blue Mouse IgG1 kappa Log in to see BC96 4
13.342041 ABIN2661003 FACS IF IHC Biotin Mouse IgG1 kappa Log in to see BC96 5
13.342041 ABIN2658042 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see BC96 5
13.342041 ABIN2660566 FACS APC-Cy7 Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2664013 FACS IF IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see BC96 4
13.342041 ABIN2657068 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2657159 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2659808 FACS IF WB PerCP Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2660057 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2659191 FACS IHC PE-Cy7 Mouse IgG1 kappa Log in to see M-A251 3
13.342041 ABIN2659471 FACS PE-Cy7 Mouse IgG1 kappa Log in to see BC96 4
13.342041 ABIN2659691 FACS PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see M-A251 3

Top referenced anti-CD25 antibody for Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 6 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 61 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 421 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 66 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 81 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(576), (453), (152), (17), (15), (9), (8), (6), (5), (4), (3), (2), (1), (1)
Host Mouse
(564), (393), (149), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Biotin
(131), (116), (92), (56), (29), (28), (28), (23), (23), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (9), (8), (8), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(915), (156), (155), (153), (109), (85), (85), (72), (48), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN1106144) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ion Exchange Chromatography.
Immunogen PHA activated T cells. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells
Clone B-B10
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN1106144) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background IL-2R (T-cell growth factor receptor) consists of a chain of 55 kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2RA is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 Molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 3559
NCBI Accession NP_000408
UniProt P01589
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN1106144) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN1106144) Images back to top
Format Liquid
Reconstitution Restore with 1.0 mL deionised water.
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted after reconstitution at 2-8 °C.

ProductDetails: References for anti-CD25 antibody (ABIN1106144)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Palmer, Farrokh-Siar, Maguire van Seventer, van Seventer: "IL-12 decreases activation-induced cell death in human naive Th cells costimulated by intercellular adhesion molecule-1. I. IL-12 alters caspase processing and inhibits enzyme function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 749-58, 2001

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN1106144) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (Biotin) (ABIN1106144) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (Biotin)