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anti-Human CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
568
457
169
19
10
10
8
5
4
3
3
2
1
1
Host
Mouse
601
406
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Biotin
130
124
100
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
936
145
141
129
127
121
80
72
48
21
18
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1106144
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30391768 ABIN1106146 FACS FITC Mouse IgG1 Log in to see B-F2 1
0.30391768 ABIN1106135 Func IHC (fro) FACS Mouse IgG1 Log in to see B-F2 1
0.30391768 ABIN1106134 Func FACS Mouse IgG1 Log in to see B-B10 18
0.30391768 ABIN1106136 FACS Mouse IgG1 Log in to see B-G3 1
0.30391768 ABIN264241 FACS PE Mouse IgG1 Log in to see 1TYV 4
0.30391768 ABIN1027672 FACS PerCP Mouse IgG1 Log in to see MEM-181 4
0.30391768 ABIN94046 FACS FITC Mouse IgG1 Log in to see MEM-181 8
0.30391768 ABIN94045 FACS Biotin Mouse IgG1 Log in to see MEM-181 8
0.30391768 ABIN94044 FACS Mouse IgG1 Log in to see MEM-181 8
0.30391768 ABIN125691 FACS APC Mouse IgG1 Log in to see MEM-181 8
0.30391768 ABIN125690 FACS PE Mouse IgG1 Log in to see MEM-181 8
0.30391768 ABIN2660756 FACS Biotin Mouse IgG1 kappa Log in to see M-A251 3
0.30391768 ABIN2661003 FACS IF IHC Biotin Mouse IgG1 kappa Log in to see BC96 5
0.30391768 ABIN2658042 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see BC96 5
0.30391768 ABIN2662232 FACS Pacific Blue Mouse IgG1 kappa Log in to see BC96 4
0.30391768 ABIN2659191 FACS IHC PE-Cy7 Mouse IgG1 kappa Log in to see M-A251 3
0.30391768 ABIN2659471 FACS PE-Cy7 Mouse IgG1 kappa Log in to see BC96 4
0.30391768 ABIN2659691 FACS PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see M-A251 3
0.30391768 ABIN2659808 FACS IF WB PerCP Mouse IgG1 kappa Log in to see M-A251 3
0.30391768 ABIN2657252 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see M-A251 3

Top referenced anti-CD25 antibody for Flow Cytometry

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Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 61 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 420 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 68 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 27 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(568), (457), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(601), (406), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Biotin
(130), (124), (100), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(936), (145), (141), (129), (127), (121), (80), (72), (48), (21), (18), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1106144) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ion Exchange Chromatography.
Immunogen PHA activated T cells. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells
Clone B-B10
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1106144) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background IL-2R (T-cell growth factor receptor) consists of a chain of 55 kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2RA is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 Molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 3559
NCBI Accession NP_000408
UniProt P01589
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1106144) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1106144) Images back to top
Format Liquid
Reconstitution Restore with 1.0 mL deionised water.
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted after reconstitution at 2-8 °C.

References for anti-CD25 Antibody (ABIN1106144)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Palmer, Farrokh-Siar, Maguire van Seventer, van Seventer: "IL-12 decreases activation-induced cell death in human naive Th cells costimulated by intercellular adhesion molecule-1. I. IL-12 alters caspase processing and inhibits enzyme function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 749-58, 2001

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1106144) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (Biotin) (ABIN1106144) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (Biotin)