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anti-Human CD25 antibody for Functional Studies

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
442
332
109
19
10
10
8
5
4
3
3
2
1
Host
Mouse
481
303
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
114
113
91
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
675
130
117
95
92
69
59
47
44
21
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1106135
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000873873 ABIN1106134 Func FACS Mouse IgG1 Log in to see B-B10 18
0.000873873 ABIN263858 Func FACS Mouse IgG1 Log in to see NR-72
0.000873873 ABIN181143 EIA Func IHC (fro) WB Mouse IgG2a Log in to see
0.000873873 ABIN1721247 Func FACS IHC Biotin Mouse IgG1 Log in to see B-B10
0.000873873 ABIN1177330 Func FACS Mouse IgG1, kappa Log in to see M-A251
0.000873873 ABIN1722531 Func FACS IHC ELISA Mouse IgG1 Log in to see B-F2
0.000873873 ABIN1722530 Func FACS IHC Mouse IgG1 Log in to see B-B10
0.000873873 ABIN3025893 Func FACS IF Mouse IgG1, kappa Log in to see 143-13
0.000873873 ABIN3025895 Func FACS IF Mouse IgG2a, kappa Log in to see IL2RA-423
0.000873873 ABIN2136292 Func FACS IHC Mouse IgG1 kappa Log in to see BC96
0.000873873 ABIN3025894 Func FACS IF Mouse IgG1, kappa Log in to see 143-13
0.000873873 ABIN3025896 Func FACS IF Mouse IgG2a, kappa Log in to see IL2RA-423

Top referenced anti-CD25 antibody for Functional Studies

  • Human Monoclonal CD25 Primary Antibody for Func, FACS - ABIN1106134 (18 Publications): Engert, Martin, Amlot, Wijdenes, Diehl, Thorpe: Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice. in International journal of cancer. Journal international du cancer 1991 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 59 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 301 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(442), (332), (109), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Mouse
(481), (303), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(114), (113), (91), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(675), (130), (117), (95), (92), (69), (59), (47), (44), (21), (17), (15), (14), (11), (10), (9), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1106135) Images
Specificity Recognizes both soluble and membrane bound Interleukin 2 Receptor a chain (IL-2 Ra), a 55 kDa protein.
Purification Ion exchange chromatography
Sterility 0.22 μm filtered
Immunogen PHA activated T cells
Clone B-F2
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1106135) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 3559
NCBI Accession NP_000408
UniProt P01589
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1106135) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1106135) Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD25 Antibody (ABIN1106135)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Engert, Martin, Amlot, Wijdenes, Diehl, Thorpe: "Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 3, pp. 450-6, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1106135) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (ABIN1106135) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody