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anti-Human CD25 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
442
332
109
19
10
10
8
5
4
3
3
2
1
Host
Mouse
481
303
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
114
113
91
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
676
130
117
95
92
70
59
46
45
21
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN114775
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089488085 ABIN114774 IHC (p) Mouse IgG1 Log in to see IL2R-1
0.00089488085 ABIN1687481 IHC (p) Rabbit IgG C-Term Log in to see SP176
0.00089488085 ABIN1449322 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
0.00089488085 ABIN1687480 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
0.00089488085 ABIN3044139 IHC (p) WB Rabbit IgG AA 39-57, N-Term Log in to see Polyclonal
0.00089488085 ABIN187250 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see IL2R-1
0.00089488085 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1
0.00089488085 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal
0.00089488085 ABIN1100566 IHC (fro) IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see
0.00089488085 ABIN1823504 ICC IHC (p) ELISA WB Mouse IgG1 AA 34-139 Log in to see 1B5D12
0.00089488085 ABIN2624041 IHC (p) WB Rabbit IgG AA 39-57 Log in to see Polyclonal
0.00089488085 ABIN1859412 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-213 Log in to see Polyclonal
0.00089488085 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal
0.00089488085 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal
0.00089488085 ABIN1905122 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
0.00089488085 ABIN384203 IHC (p) Mouse IgG2b Log in to see 4C9
0.00089488085 ABIN609296 IHC (fro) IHC (p) Mouse Log in to see
0.00089488085 ABIN384204 IHC (p) Mouse IgG2b Log in to see 4C9
0.00089488085 ABIN951245 IHC (p) Mouse IgG2b Log in to see 4C9
0.00089488085 ABIN384205 IHC (p) Mouse IgG2b Log in to see 4C9

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 59 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 301 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(442), (332), (109), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Mouse
(481), (303), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(114), (113), (91), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(676), (130), (117), (95), (92), (70), (59), (46), (45), (21), (17), (15), (14), (11), (10), (9), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha).
Cellular Localization: Cell membrane.
Purification Purified
Immunogen BALB/C mice were injected with recombinant human interleukin-2 receptor.
Clone IL2R-1
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages. The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 3559
NCBI Accession NP_000408
UniProt P01589
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Immunohistochemistry on Paraffin Sections: 1/25-1/75 in an ABC method (30 minutes atroom temperature)Recommended Positive Control: Tonsil. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (ABIN114775) Formalin fixed paraffin embedded human tonsil stained with CD25 antibody. ABIN114774