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anti-Human CD25 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human, Mouse (Murine)
556
437
152
17
14
9
8
4
4
3
3
2
1
1
Host
Mouse
553
379
141
7
5
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
126
112
90
54
29
27
26
23
22
22
17
16
12
11
11
11
11
11
10
8
8
8
8
8
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
882
153
149
142
108
82
77
68
45
21
19
17
15
14
13
12
9
7
6
6
5
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN153451
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.1594715 ABIN4260055 IHC (p) Rabbit IgG Log in to see EP218
12.1594715 ABIN4291007 IHC IHC (p) WB Rabbit C-Term Log in to see SP176
1 ABIN114775 IHC (p) Mouse IgG1 Log in to see IL2R-1
1 ABIN114774 IHC (p) Mouse IgG1 Log in to see IL2R-1
1 ABIN1449323 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
1 ABIN1687481 IHC (p) Rabbit IgG C-Term Log in to see SP176
1 ABIN1687480 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
1 ABIN3044139 IHC (p) WB Rabbit IgG AA 39-57, N-Term Log in to see Polyclonal
1 ABIN187250 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see IL2R-1
1 ABIN1100566 IHC (fro) IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see
1 ABIN4291008 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 kappa Log in to see 2R12
1 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal
1 ABIN1823504 ICC IHC (p) ELISA WB Mouse IgG1 AA 34-139 Log in to see 1B5D12
1 ABIN2624041 IHC (p) WB Rabbit IgG AA 39-57 Log in to see Polyclonal
1 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal
1 ABIN1905122 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
1 ABIN4291002 IHC IHC (p) Rabbit Log in to see Polyclonal
1 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal
1 ABIN951245 IHC (p) Mouse IgG2b Log in to see 4C9
1 ABIN609296 IHC (fro) IHC (p) Mouse Log in to see

Top referenced anti-CD25 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 17 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 367 Antibodies
Functional Studies (Func) 26 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human, Mouse (Murine)
(556), (437), (152), (17), (14), (9), (8), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(553), (379), (141), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(126), (112), (90), (54), (29), (27), (26), (23), (22), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(882), (153), (149), (142), (108), (82), (77), (68), (45), (21), (19), (17), (15), (14), (13), (12), (9), (7), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN153451) Images
Specificity This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cells, HTLV-1 or HTLV-2 positive T cell lines. It is moderately expressed on activated B cells, NK-cells, and macrophages.
Purification Protein A or G purified
Immunogen BALB/C mice were injected with recombinant human interleukin-2 receptor.
Clone IL2R-1
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN153451) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Gene Symbol: IL2RA
Gene ID 3559
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN153451) Images back to top
Application Notes Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:75, Immunohistochemistry-Frozen 1:10-1:500Immunohistochemistry: use for 30 mins (ABC method) on pareffin-embedded sections. Use on frozen sections was reported in scientific literature (PMID: 22665223). Optimal dilution should be determined by the end user. Formalin fixed paraffin embedded sections require high temperature antigen retrieval unmasking with 10 mM citrate buffer, pH 6.0 prior to staining. Has been reported to work in ICC/IF, mouse (PMID:22009917)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN153451) Images back to top
Format Liquid
Buffer Purified immnoglobulin fraction in PBS with 2 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-CD25 Antibody (ABIN153451)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Grati, Yan, Raval, Walsh, Ma, Chakchouk, Kannan-Sundhari, Mittal, Masmoudi, Blanton, Tekin, King, Yengo, Liu: "MYO3A Causes Human Dominant Deafness and Interacts with Protocadherin 15-CD2 Isoform." in: Human mutation, Vol. 37, Issue 5, pp. 481-7, 2016 Further details: Immunocytochemistry,Immunofluorescence

Liang, Gao, Lin, Goss, Du, Li: "Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model." in: Oncogene, Vol. 34, Issue 33, pp. 4368-78, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Sorrentino, Avellini, Geraci, Vadalà: "Pre-clinical Crohn's disease: diagnosis, treatment and six year follow-up." in: Journal of Crohn's & colitis, Vol. 8, Issue 7, pp. 702-7, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Joo, Kim, Choi, Kim, Kim, Moon: "Detection of prostaglandin E2-induced dendritic cell migration into the lymph nodes of mice using a 1.5 T clinical MR scanner." in: NMR in biomedicine, Vol. 25, Issue 4, pp. 570-9, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Campbell-Thompson, Heiple, Montgomery, Zhang, Schneider: "Staining protocols for human pancreatic islets." in: Journal of visualized experiments : JoVE, Issue 63, pp. e4068, 2012 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN153451) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (ABIN153451) Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC method.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (ABIN153451) Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (IL2R.1) [ABIN153451] - Hum...