We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Biochemical Assay (BCA)||4 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||301 Antibodies|
|Functional Studies (Func)||13 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||55 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||18 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||27 Antibodies|
|Immunoprecipitation (IP)||26 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling Images
This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha).
Cellular Localization: Cell membrane.
|Immunogen||BALB/C mice were injected with recombinant human interleukin-2 receptor.|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages. The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Immunohistochemistry on Paraffin Sections: 1/25-1/75 in an ABC method (30 minutes atroom temperature)Recommended Positive Control: Tonsil. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top