anti-Human CD25 antibody for Neutralization

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
AA 22-213
22
20
20
18
15
15
14
13
13
7
6
6
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
576
453
152
17
15
9
8
6
5
4
3
2
1
1
Host
Goat
565
393
149
7
4
3
2
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
131
116
93
56
29
28
28
23
23
22
17
16
12
11
11
11
11
11
10
9
8
8
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
915
156
155
152
109
85
85
72
48
21
18
17
16
14
13
12
9
7
6
6
6
5
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN4899652
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2664435 ICFC Neut Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN2664436 ICFC Neut Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN4291070 BP CyTOF FACS IF IHC Neut WB Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
1 ABIN4291069 BP CyTOF FACS IF IHC Neut WB Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
1 ABIN4291068 BP CyTOF FACS Neut Rabbit IgG Log in to see Daclizumab
1 ABIN4291067 BP CyTOF FACS Neut Rabbit IgG Log in to see Daclizumab
1 ABIN1983279 Neut IHC WB Mouse IgG1 AA 22-213 Log in to see MM0391-1X27
1 ABIN2224527 FACS Neut ELISA Mouse IgG1 kappa Log in to see 5J69
1 ABIN2850774 Neut ELISA WB HRP Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2850575 Neut ELISA WB Alkaline Phosphatase (AP) Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2850624 Neut ELISA WB APC Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2850724 Neut ELISA WB FITC Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2850674 Neut ELISA WB Biotin Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2850824 Neut ELISA WB PE Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2288540 Neut ELISA WB Mouse IgG2a Soluble Log in to see 7F4
1 ABIN2471751 Neut IHC ELISA WB Mouse IgG2a kappa Log in to see
1 ABIN1724604 IHC (p) Neut ELISA WB Mouse IgG2a kappa Log in to see

Top referenced anti-CD25 antibody for Neutralization

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 6 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 61 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 421 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 66 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 81 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 22-213
(22), (20), (20), (18), (15), (15), (14), (13), (13), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(576), (453), (152), (17), (15), (9), (8), (6), (5), (4), (3), (2), (1), (1)
Host Goat
(565), (393), (149), (7), (4), (3), (2), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(131), (116), (93), (56), (29), (28), (28), (23), (23), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (9), (8), (8), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(915), (156), (155), (152), (109), (85), (85), (72), (48), (21), (18), (17), (16), (14), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN4899652) Images
Specificity Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15 % cross-reactivity with recombinant human (rh) IL-2 sR gamma is observed, less than 5 % cross-reactivity with rhIL-6 sR is observed, and less than 1 % cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5 % cross-reactivity with recombinant mouse IL-2 R alpha is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha , Glu22-Cys213, Accession # P01589
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN4899652) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Alternative Names: CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene ID 3559
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN4899652) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Neutralization 0.2-1 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN4899652) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-CD25 antibody (ABIN4899652)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Zheng, Jones, Shi, Wiseman, Marr, Berenger, Huston, Gill, Krensky, Kubes, Mody: "Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7221-9, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Binder, Vögtle, Michelfelder, Müller, Illerhaus, Sundararajan, Mertelsmann, Trepel: "Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab." in: Cancer research, Vol. 67, Issue 8, pp. 3518-23, 2007 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Li, Ma, Wang, Li: "Interleukin-2alpha receptor in membrane lipid rafts." in: Transplantation proceedings, Vol. 37, Issue 5, pp. 2395-7, 2005 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sandrin, Muriaux, Darlix, Cosset: "Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses." in: Journal of virology, Vol. 78, Issue 13, pp. 7153-64, 2004 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN4899652) back to top
Supplier Images
Neutralization (Neut) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 22-213) (ABIN4899652) Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 R alpha Anti...
Neutralization (Neut) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 22-213) (ABIN4899652) Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 R alpha Anti...