anti-Human CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
pSer268
22
20
19
18
15
15
14
13
13
7
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
561
444
152
17
15
9
8
6
5
4
3
2
1
1
Host
Rabbit
558
384
142
7
5
3
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
128
114
91
54
29
28
27
23
22
22
17
16
12
11
11
11
11
11
10
8
8
8
8
8
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
896
154
148
147
107
83
83
68
46
21
19
17
16
14
13
12
9
7
6
6
5
4
3
2
2
1
1
1
1
Options
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Catalog No. ABIN1531665
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.157866 ABIN1532689 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.157866 ABIN1724861 ICC IHC ELISA WB Mouse IgG1 AA 34-139 Log in to see 1B5D12 2
3.157866 ABIN3044139 IHC (p) WB Rabbit IgG AA 39-57, N-Term Log in to see Polyclonal
3.157866 ABIN1687480 IHC (p) WB Rabbit IgG C-Term Log in to see SP176
3.157866 ABIN3187774 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.157866 ABIN2705770 WB Rabbit Center Log in to see Polyclonal
3.157866 ABIN498207 IF WB Rabbit Log in to see Polyclonal
3.157866 ABIN1539791 WB Mouse IgG2a Log in to see 7G7B6
3.157866 ABIN1873210 WB Rabbit IgG Log in to see Polyclonal
3.157866 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12
3.157866 ABIN439058 ICC IF WB Rabbit Log in to see Polyclonal
3.157866 ABIN4291007 IHC IHC (p) WB Rabbit C-Term Log in to see SP176
3.157866 ABIN1859412 ICC IHC IP WB Rabbit IgG AA 22-213 Log in to see Polyclonal
3.157866 ABIN3031471 ELISA WB Mouse IgG2a Log in to see 7G7B6
3.157866 ABIN561456 ELISA WB Mouse IgG2a kappa AA 22-122, partial Log in to see 1D6
3.157866 ABIN948021 WB Mouse AA 1-272, full length Log in to see Polyclonal
3.157866 ABIN517010 WB Rabbit AA 1-272, full length Log in to see Polyclonal
3.157866 ABIN1845343 ELISA IF/ICC IHC WB Mouse IgG1 AA 34-139 Log in to see
3.157866 ABIN3031470 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.157866 ABIN2426093 ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-CD25 antibody for Western Blotting

  • Human Polyclonal CD25 Primary Antibody for FACS, Neut - ABIN4899652 (4 Publications): Binder, Vögtle, Michelfelder, Müller, Illerhaus, Sundararajan, Mertelsmann, Trepel: Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab. in Cancer research 2007 (PubMed)

  • Human Monoclonal CD25 Primary Antibody for FACS, WB - ABIN4899653 (3 Publications): Voo, Peng, Guo, Fu, Li, Frappier, Wang: Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation. in Cancer research 2005 (PubMed)

  • Human Monoclonal CD25 Primary Antibody for ICC, IHC - ABIN1724861 (2 Publications): Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients. in Annals of hematology 2012 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 60 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 410 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope pSer268
(22), (20), (19), (18), (15), (15), (14), (13), (13), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(561), (444), (152), (17), (15), (9), (8), (6), (5), (4), (3), (2), (1), (1)
Host Rabbit
(558), (384), (142), (7), (5), (3), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(128), (114), (91), (54), (29), (28), (27), (23), (22), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(896), (154), (148), (147), (107), (83), (83), (68), (46), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN1531665) Images
Specificity IL-2R alpha/CD25 (Phospho-Ser268) Antibody detects endogenous levels of IL-2R alpha/CD25 only when phosphorylated at Ser268.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25 around the phosphorylation site of Ser268.
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN1531665) Images back to top
Antigen
Alternative Name IL-2R alpha/cd25 (IL2RA Antibody Abstract)
Background Molecular weight of target protein: 30 kDa
Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen, CD25
Molecular Weight 30 kDa
Gene ID 3559
OMIM 147730
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN1531665) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.231367 (NCBI Gene Symbol: IL2RA)

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN1531665) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-CD25 antibody (ABIN1531665)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Bamborough, Hedgecock, Richards: "The interleukin-2 and interleukin-4 receptors studied by molecular modelling." in: Structure (London, England : 1993), Vol. 2, Issue 9, pp. 839-51, 1995

Nikaido, Shimizu, Ishida, Sabe, Teshigawara, Maeda, Uchiyama, Yodoi, Honjo: "Molecular cloning of cDNA encoding human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 631-5, 1984

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN1531665) back to top
Supplier Images
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (pSer268) (ABIN1531665) Western blot analysis of extracts from LOVO cells, using IL-2R alpha/CD25 (Phospho-Se...
Immunofluorescence (IF) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (pSer268) (ABIN1531665) Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 (Phospho-Ser268) An...