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anti-Mouse (Murine) CD25 antibody for ELISA

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
AA 34-139
22
20
15
13
12
11
7
7
7
6
5
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
576
461
169
19
10
10
8
4
4
3
3
2
1
1
Host
Mouse
608
411
135
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
125
102
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
948
149
141
131
129
125
84
74
48
21
19
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1724861
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32693866 ABIN1845343 ICC IF IHC ELISA WB Mouse IgG1 AA 34-139 Log in to see
0.0012117105 ABIN1584197 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN2490712 IF ELISA WB Rabbit IgG Ser268 Log in to see Polyclonal
0.0012117105 ABIN2689117 CyTox IHC (fro) FACS IP ELISA Rat IgM, kappa Log in to see 7D4 15
0.0012117105 ABIN2490713 IF ELISA WB Rabbit IgG pSer268 Log in to see Polyclonal
0.0012117105 ABIN2000717 ELISA Rabbit IgG AA 1-236 Log in to see Polyclonal 2
0.0012117105 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0012117105 ABIN2624038 ELISA WB Biotin Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0012117105 ABIN2426093 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2609826 ELISA Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0012117105 ABIN2431535 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12
0.0012117105 ABIN2000715 ELISA Rabbit AA 1-236 Log in to see
0.0012117105 ABIN2692464 ELISA Rabbit Log in to see Polyclonal

Top referenced anti-CD25 antibody for ELISA

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies
ELISA 65 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 136 Antibodies
Functional Studies (Func) 7 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 60 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 41 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 34-139
(22), (20), (15), (13), (12), (11), (7), (7), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(576), (461), (169), (19), (10), (10), (8), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(608), (411), (135), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (125), (102), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(948), (149), (141), (131), (129), (125), (84), (74), (48), (21), (19), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images
Purification Purified
Immunogen Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Clone 1B5D12
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight 30.8 kDa
Gene ID 3559
HGNC 3559
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1724861) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD25 Antibody (ABIN1724861)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) back to top
Supplier Images
ELISA image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
Immunocytochemistry (ICC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5)