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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||16 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Flow Cytometry (FACS)||280 Antibodies|
|Functional Studies (Func)||25 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||90 Antibodies|
|In vitro Assay (in vitro)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||17 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD25 antibody is conjugated to PE Alternatives|
|7 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2663213) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2663213) Images back to top
|Alternative Name||CD25 (IL2RA Antibody Abstract)|
|Background||CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2663213) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2663213) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD25 Antibody (ABIN2663213)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Lu, Wang, Yan et al.: "Liver TCRγδ(+) CD3(+) CD4(-) CD8(-) T cells contribute to murine hepatitis virus strain 3-induced hepatic injury through a TNF-α-dependent pathway." in: Molecular immunology, Vol. 52, Issue 3-4, pp. 229-36, 2012 (PubMed).
Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000 (PubMed).
Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).
Ortega, Robb, Shevach et al.: "The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1970-5, 1984 (PubMed).
Lowenthal, Corthésy, Tougne et al.: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985 (PubMed).
Moreau, Nabholz, Diamantstein et al.: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987 (PubMed).
Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991 (PubMed).
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2663213) back to top