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anti-Mouse (Murine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
575
461
170
19
10
10
8
6
4
3
3
2
1
1
Host
Rat
608
411
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Biotin
131
125
101
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
949
149
142
133
131
125
81
75
48
21
21
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN135173
$ 295.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3087334 ABIN951248 FACS FITC Rat IgG1 Log in to see PC61-5-3
0.3087334 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3
0.3087334 ABIN2657371 FACS Alexa Fluor 488 Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2658857 FACS APC Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2658949 FACS IP IHC PE-Cy5 Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2658552 FACS IF IHC APC Rat IgG2b kappa Log in to see 3C7 8
0.3087334 ABIN2662234 FACS Pacific Blue Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2657710 FACS CyTOF Alexa Fluor 647 Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2659693 FACS PE/Dazzle™ 594 Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2661005 FACS Biotin Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN2665963 FACS Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN370886 FACS PE Rat IgM Log in to see 7D4
0.3087334 ABIN2664438 FACS Rat IgG1 lambda Log in to see PC61 12
0.3087334 ABIN262337 FACS IHC (fro) IHC Rat IgG2b kappa Log in to see 3C7
0.3087334 ABIN4260058 FACS in vivo Rat Log in to see PC61 1
0.3087334 ABIN2678216 FACS PE-Cy5.5 Rat IgG Mix lambda Log in to see PC61-5
0.3087334 ABIN2678220 FACS EFluor 450 Rat IgG2b kappa Log in to see 3C7 (3C7)
0.3087334 ABIN2678218 FACS EFluor 450 Rat IgG Mix lambda Log in to see PC61-5
0.3087334 ABIN2678224 FACS PE-eFluor® 610 Rat IgG Mix lambda Log in to see PC61-5
0.3087334 ABIN2678222 FACS EFluor 660 Rat IgM kappa Log in to see 7D4 (7D4)

Top referenced anti-CD25 antibody for Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 16 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 397 Antibodies
Functional Studies (Func) 28 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 52 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 84 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(575), (461), (170), (19), (10), (10), (8), (6), (4), (3), (3), (2), (1), (1)
Host Rat
(608), (411), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Biotin
(131), (125), (101), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(949), (149), (142), (133), (131), (125), (81), (75), (48), (21), (21), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN135173) Images
Characteristics The antibody detects: Low affinity α chain IL-2 receptor (IL-2Rα), Mr 55 kDa.
The monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody. While the monoclonal antibody does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
To insure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to charactersistics of a standard reference reagent.
Immunogen IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Clone 7D4
Isotype IgM

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN135173) Images back to top
Antigen
Alternative Name CD25/IL-2Ralpha (IL2RA Antibody Abstract)
Background The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN135173) Images back to top
Application Notes Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN135173) Images back to top
Concentration 0,5 mg/mL
Buffer PBS / NaN3
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C

References for anti-CD25 Antibody (ABIN135173)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Lorenzo, Jaulin, Vita, Froussard, Ferrara, Jankovic, Theze: "Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2970-7, 1991

Malek, Robb, Shevach: "Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5694-8, 1983

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN135173) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (Biotin) (ABIN135173) Rat Anti-Mouse CD25-R-PE. Amount used: 0.1 ug/10^6 cells. Cells from BALB/c spleen we...