anti-Mouse (Murine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
AA 22-236
22
21
20
17
15
15
14
13
13
7
6
6
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
577
452
152
17
15
9
8
6
5
4
3
2
1
1
Host
Rat
565
392
149
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Allophycocyanin
131
116
93
56
29
28
28
23
23
21
17
16
12
11
11
11
11
11
10
9
8
8
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
915
156
155
153
109
85
85
72
48
21
19
17
16
14
13
12
9
7
6
6
6
5
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN4896465
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.317171 ABIN4896466 FACS Allophycocyanin Rat IgG2a AA 22-236 Log in to see 280406 1
13.317171 ABIN4260058 CyTOF FACS in vivo Rat IgG Mix lambda Log in to see PC61 1
13.317171 ABIN135173 FACS IP Biotin Rat IgM Log in to see 7D4 2
13.317171 ABIN951248 FACS FITC Rat IgG1 Log in to see PC61-5-3
13.317171 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3
13.317171 ABIN2661005 FACS Biotin Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2659693 FACS PE/Dazzle™ 594 Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2657710 FACS CyTOF Alexa Fluor 647 Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2658552 FACS IF IHC APC Rat IgG2b kappa Log in to see 3C7 8
13.317171 ABIN2657371 FACS Alexa Fluor 488 Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2662234 FACS Pacific Blue Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2658949 FACS IP IHC PE-Cy5 Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2658857 FACS APC Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN2665963 FACS Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN370886 FACS PE Rat IgM Log in to see 7D4
13.317171 ABIN2664438 FACS Rat IgG1 lambda Log in to see PC61 12
13.317171 ABIN4896467 FACS PerCP Rat IgG2a AA 22-236 Log in to see 280406
13.317171 ABIN4896468 FACS PerCP Rat IgG2a AA 22-236 Log in to see 280406
13.317171 ABIN4896469 FACS Alexa Fluor 488 Rat IgG2a AA 22-236 Log in to see 280406
13.317171 ABIN4896470 FACS Alexa Fluor 488 Rat IgG2a AA 22-236 Log in to see 280406

Top referenced anti-CD25 antibody for Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 17 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 382 Antibodies
Functional Studies (Func) 30 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 22-236
(22), (21), (20), (17), (15), (15), (14), (13), (13), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(577), (452), (152), (17), (15), (9), (8), (6), (5), (4), (3), (2), (1), (1)
Host Rat
(565), (392), (149), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Allophycocyanin
(131), (116), (93), (56), (29), (28), (28), (23), (23), (21), (17), (16), (12), (11), (11), (11), (11), (11), (10), (9), (8), (8), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(915), (156), (155), (153), (109), (85), (85), (72), (48), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN4896465) Images
Specificity Detects mouse CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) gamma c, recombinant human CD25/IL-2 R alpha , rmIL-2 R beta , or rmIL-15 R is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha , Glu22-Lys236, Accession # P01590
Clone 280406
Isotype IgG2a

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN4896465) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Alternative Names: CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene ID 3559
UniProt P01590
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN4896465) Images back to top
Application Notes Flow Cytometry 10 uL/10^6 cells
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN4896465) Images back to top
Format Liquid
Buffer Supplied in a saline solution containing BSA and Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Protect from light. Do not freeze. 12 months from date of receipt,2°C to 8°C as supplied.
Expiry Date 12 months

ProductDetails: References for anti-CD25 antibody (ABIN4896465)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Llopiz, Dotor, Casares, Bezunartea, Díaz-Valdés, Ruiz, Aranda, Berraondo, Prieto, Lasarte, Borrás-Cuesta, Sarobe: "Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy." in: International journal of cancer, Vol. 125, Issue 11, pp. 2614-23, 2009 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN4896465) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 22-236) (Allophycocyanin) (ABIN4896465) Detection of CD25/IL-2 R alpha in CD4+ Mouse T cells by Flow Cytometry. CD4+ mouse T ...
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 22-236) (Allophycocyanin) (ABIN4896465) Detection of CD25/IL-2 R alpha in CD4+ Mouse T cells by Flow Cytometry. CD4+ mouse T ...