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anti-Mouse (Murine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
569
456
169
19
10
10
8
5
4
3
3
2
1
1
Host
Rat
602
405
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to FITC
130
123
101
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
936
145
141
129
127
121
80
72
48
21
18
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN951248
$ 423.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30464333 ABIN135173 FACS IP Biotin Rat IgM Log in to see 7D4 2
0.30464333 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3
0.30464333 ABIN2658857 FACS APC Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2658949 FACS IP IHC PE-Cy5 Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2659693 FACS PE/Dazzle™ 594 Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2661005 FACS Biotin Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2657710 FACS CyTOF Alexa Fluor 647 Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2658552 FACS IF IHC APC Rat IgG2b kappa Log in to see 3C7 8
0.30464333 ABIN2657371 FACS Alexa Fluor 488 Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2662234 FACS Pacific Blue Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN2665963 FACS Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN370886 FACS PE Rat IgM Log in to see 7D4
0.30464333 ABIN2664438 FACS Rat IgG1 lambda Log in to see PC61 12
0.30464333 ABIN262337 FACS IHC (fro) IHC Rat IgG2b kappa Log in to see 3C7
0.30464333 ABIN2678218 FACS EFluor 450 Rat IgG Mix lambda Log in to see PC61-5
0.30464333 ABIN2678220 FACS EFluor 450 Rat IgG2b kappa Log in to see 3C7 (3C7)
0.30464333 ABIN2678224 FACS PE-eFluor® 610 Rat IgG Mix lambda Log in to see PC61-5
0.30464333 ABIN2678222 FACS EFluor 660 Rat IgM kappa Log in to see 7D4 (7D4)
0.30464333 ABIN2678216 FACS PE-Cy5.5 Rat IgG Mix lambda Log in to see PC61-5
0.30464333 ABIN2168764 FACS SPRD Rat IgM Log in to see 7D4

Top referenced anti-CD25 antibody for Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 15 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 403 Antibodies
Functional Studies (Func) 28 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 17 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(569), (456), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Rat
(602), (405), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to FITC
(130), (123), (101), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(936), (145), (141), (129), (127), (121), (80), (72), (48), (21), (18), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity This monoclonal antibody reacts with the low affinity alpha chain of the Interleukin-2 receptor antigen present on activated T and B cells in mice. AM26037FC inhibits IL-2 binding and IL-2 dependent proliferation.
Purification Affinity chromatography on Protein G
Immunogen B6.1 CTL Cell line Donor: OFA rat spleen Fusion Partner: mouse myeloma line P3X63Ag8.653
Clone PC61-5-3
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 16184
NCBI Accession NP_032393
UniProt P01590
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Concentration 0,1 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (FITC) (ABIN951248) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (FITC)