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anti-Mouse (Murine) CD25 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
577
461
170
19
10
10
8
5
4
3
3
2
1
1
Host
Rat
609
410
135
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Biotin
131
125
101
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
947
148
142
132
130
124
84
74
48
21
20
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN951247
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN1302515 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5 8
0.000883884 ABIN2689121 IHC (fro) FACS IHC (p) Biotin Rat IgM, kappa Log in to see 7D4 6
0.000883884 ABIN1302435 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5 6
0.000883884 ABIN1302633 Func IHC (fro) FACS IP APC Rat IgG1 Log in to see PC61-5 8
0.000883884 ABIN1302926 Func IHC (fro) FACS IP FITC Rat IgG1 Log in to see PC61-5 8
0.000883884 ABIN1303127 Func IHC (fro) FACS IP PE Rat IgG1 Log in to see PC61-5 8
0.000883884 ABIN262337 FACS IHC (fro) IHC Rat IgG2b kappa Log in to see 3C7
0.000883884 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1 5
0.000883884 ABIN2689117 CyTox IHC (fro) FACS IP ELISA Rat IgM, kappa Log in to see 7D4 15
0.000883884 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
0.000883884 ABIN2479486 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
0.000883884 ABIN601655 IHC (fro) FACS IP Rat IgG1 Log in to see
0.000883884 ABIN601656 IHC (fro) FACS IP Rat IgG1 Log in to see
0.000883884 ABIN601654 Func IHC (fro) FACS IP Rat IgG1 Log in to see
0.000883884 ABIN2479481 IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
0.000883884 ABIN3200888 FACS Func IHC (fro) IP PE Rat IgG1 Log in to see PC61-5
0.000883884 ABIN3199663 FACS Func IHC (fro) IP APC Rat IgG1 Log in to see PC61-5
0.000883884 ABIN3199664 FACS Func IHC (fro) IP FITC Rat IgG1 Log in to see PC61-5
0.000883884 ABIN153558 FACS IHC IHC (fro) IHC (p) IP Rat IgG1 Log in to see PC61-5-3-
0.000883884 ABIN1819631 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3

Top referenced anti-CD25 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 15 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 403 Antibodies
Functional Studies (Func) 28 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(577), (461), (170), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Rat
(609), (410), (135), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Biotin
(131), (125), (101), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(947), (148), (142), (132), (130), (124), (84), (74), (48), (21), (20), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity Anti-mouse CD25 (IL-2R) monoclonal antibody reacts with the low affinity alpha chain of the interleukin-2 receptor antigen present on activated T and B cells in mice. CL8925B inhibits IL-2 binding and IL-2 dependent proliferation.
Purification Affinity chromatography on Protein G
Immunogen B6.1 CTL Cell line Donor: OFA rat spleen Fusion Partner: Mouse myeloma line P3X63Ag8.653
Clone PC61-5-3
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 16184
NCBI Accession NP_032393
UniProt P01590
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Concentration 0,1 mg/mL
Buffer PBS containing 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (Biotin) (ABIN951247) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (Biotin)