anti-Mouse (Murine) CD25 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human, Mouse (Murine)
590
471
166
22
19
15
9
8
6
6
4
4
3
3
2
1
Host
Mouse
589
398
151
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
118
95
56
30
29
28
24
23
22
18
16
14
12
12
12
12
12
10
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
932
186
168
156
125
89
84
82
61
28
21
21
19
17
13
12
9
7
6
6
6
5
3
2
1
1
1
1
Supplier
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Catalog No. ABIN153451
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3
1 ABIN153558 CyTOF FACS IHC IHC (fro) IHC (p) IP Rat IgG1 Log in to see PC61-5-3
1 ABIN262337 FACS IHC (fro) IHC Rat IgG2b kappa Log in to see 3C7
1 ABIN2689121 IHC (fro) FACS IHC (p) Biotin Rat IgM, kappa Log in to see 7D4 6
1 ABIN2479481 IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
1 ABIN2479486 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
1 ABIN2689117 CyTox IHC (fro) FACS IP ELISA Rat IgM, kappa Log in to see 7D4 15
1 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
1 ABIN4290998 ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see IL2R-1
1 ABIN3200888 FACS Func IHC (fro) IP PE Rat IgG1 Log in to see PC61-5
1 ABIN601656 IHC (fro) FACS IP Rat IgG1 Log in to see
1 ABIN601655 IHC (fro) FACS IP Rat IgG1 Log in to see
1 ABIN3199663 FACS Func IHC (fro) IP APC Rat IgG1 Log in to see PC61-5
1 ABIN3199661 FACS Func IHC (fro) IP Rat IgG1 Log in to see PC61-5
1 ABIN3199664 FACS Func IHC (fro) IP FITC Rat IgG1 Log in to see PC61-5
1 ABIN1819631 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3
1 ABIN601654 Func IHC (fro) FACS IP Rat IgG1 Log in to see
1 ABIN3199662 FACS Func IHC (fro) IP Rat IgG1 Log in to see PC61-5
1 ABIN5540138 FACS Func IHC (fro) IP Rat IgG1 Log in to see PC61-5-3
1 ABIN5580834 FACS Func IHC (fro) IP PE Rat IgG1 Log in to see PC61-5

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human, Mouse (Murine)
(590), (471), (166), (22), (19), (15), (9), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(589), (398), (151), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (118), (95), (56), (30), (29), (28), (24), (23), (22), (18), (16), (14), (12), (12), (12), (12), (12), (10), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(932), (186), (168), (156), (125), (89), (84), (82), (61), (28), (21), (21), (19), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN153451) Images
Specificity This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cells, HTLV-1 or HTLV-2 positive T cell lines. It is moderately expressed on activated B cells, NK-cells, and macrophages.
Purification Protein A or G purified
Immunogen BALB/C mice were injected with recombinant human interleukin-2 receptor.
Clone IL2R-1
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN153451) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Gene Symbol: IL2RA
Gene ID 3559
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN153451) Images back to top
Application Notes Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:75, Immunohistochemistry-Frozen 1:10-1:500Immunohistochemistry: use for 30 mins (ABC method) on pareffin-embedded sections. Use on frozen sections was reported in scientific literature (PMID: 22665223). Optimal dilution should be determined by the end user. Formalin fixed paraffin embedded sections require high temperature antigen retrieval unmasking with 10 mM citrate buffer, pH 6.0 prior to staining. Has been reported to work in ICC/IF, mouse (PMID:22009917)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN153451) Images back to top
Format Liquid
Buffer Purified immnoglobulin fraction in PBS with 2 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-CD25 antibody (ABIN153451)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Grati, Yan, Raval, Walsh, Ma, Chakchouk, Kannan-Sundhari, Mittal, Masmoudi, Blanton, Tekin, King, Yengo, Liu: "MYO3A Causes Human Dominant Deafness and Interacts with Protocadherin 15-CD2 Isoform." in: Human mutation, Vol. 37, Issue 5, pp. 481-7, 2016 Further details: Immunocytochemistry,Immunofluorescence

Liang, Gao, Lin, Goss, Du, Li: "Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model." in: Oncogene, Vol. 34, Issue 33, pp. 4368-78, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Sorrentino, Avellini, Geraci, Vadalà: "Pre-clinical Crohn's disease: diagnosis, treatment and six year follow-up." in: Journal of Crohn's & colitis, Vol. 8, Issue 7, pp. 702-7, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Joo, Kim, Choi, Kim, Kim, Moon: "Detection of prostaglandin E2-induced dendritic cell migration into the lymph nodes of mice using a 1.5 T clinical MR scanner." in: NMR in biomedicine, Vol. 25, Issue 4, pp. 570-9, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Campbell-Thompson, Heiple, Montgomery, Zhang, Schneider: "Staining protocols for human pancreatic islets." in: Journal of visualized experiments : JoVE, Issue 63, pp. e4068, 2012 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN153451) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN153451) Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC method.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN153451) Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (IL2R.1) [ABIN153451] - Hum...