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anti-Mouse (Murine) CD25 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
443
331
109
19
10
10
8
5
4
3
3
2
1
Host
Rat
482
302
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Biotin
114
113
90
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
675
130
117
95
92
69
59
46
45
21
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2689121
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089724595 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1
0.00089724595 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal
0.00089724595 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
0.00089724595 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal
0.00089724595 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal
0.00089724595 ABIN438068 FACS IHC (p) IHC Rat IgG1 Log in to see AP-MAB0710

Top referenced anti-CD25 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 14 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 280 Antibodies
Functional Studies (Func) 25 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 17 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(443), (331), (109), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Rat
(482), (302), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Biotin
(114), (113), (90), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(675), (130), (117), (95), (92), (69), (59), (46), (45), (21), (17), (15), (14), (11), (10), (9), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2689121) Images
Brand BD Pharmingen™
Characteristics The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks binding of IL-2 to CD25. Immunohistochemical staining of CD25 positive cells. Frozen sections of mouse thymus were reacted with the anti-CD25 antibody. Immature thymocytes expressing the IL-2 receptor can be identified by the brown labeling of their cell membranes. Amplification 20x.

BD Pharmingen™ Biotin Rat Anti-Mouse CD25 - Biotin - Clone 7D4 - Isotype Rat IgM, κ - Reactivity Ms - 1 mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Clone 7D4
Isotype IgM, kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2689121) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background Synonyms: IL-2 Receptor α chain, p55
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2689121) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2689121) Images back to top
Format Liquid
Concentration 125 μg/mL
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD25 Antibody (ABIN2689121)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, 1990

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989

Habu, Okumura, Diamantstein, Shevach: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985

Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985