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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||16 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Flow Cytometry (FACS)||280 Antibodies|
|Functional Studies (Func)||25 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||90 Antibodies|
|In vitro Assay (in vitro)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||17 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD25 antibody is conjugated to Biotin Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|6 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2689121) Images
The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks binding of IL-2 to CD25. Immunohistochemical staining of CD25 positive cells. Frozen sections of mouse thymus were reacted with the anti-CD25 antibody. Immature thymocytes expressing the IL-2 receptor can be identified by the brown labeling of their cell membranes. Amplification 20x.
BD Pharmingen™ Biotin Rat Anti-Mouse CD25 - Biotin - Clone 7D4 - Isotype Rat IgM, κ - Reactivity Ms - 1 mL
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||IL-2-dependent BALB/c mouse helper T-cell clone HT-2|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2689121) Images back to top
|Alternative Name||CD25 (IL2RA Antibody Abstract)|
|Background||Synonyms: IL-2 Receptor α chain, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2689121) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2689121) Images back to top
|Buffer||Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-CD25 Antibody (ABIN2689121)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
|Product cited in:||
Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).
Chen, Ma, Young et al.: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995 (PubMed).
Habu, Okumura, Diamantstein et al.: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985 (PubMed).
Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985 (PubMed).
Crowley, Inaba, Witmer-Pack et al.: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989 (PubMed).
Garni-Wagner, Witte, Tutt et al.: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, 1990 (PubMed).