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anti-Rat (Rattus) CD25 antibody for ELISA (Detection)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Rat (Rattus)
557
438
151
17
14
9
8
4
4
3
3
2
1
1
Host
Mouse
553
379
141
7
5
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Alexa Fluor 488
126
112
90
54
28
27
26
23
22
22
17
16
12
11
11
11
11
11
10
8
8
8
8
8
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Detection), Flow Cytometry (FACS), Immunohistochemistry (IHC)
881
153
149
142
108
83
78
69
45
21
19
17
16
14
13
12
9
7
6
5
5
4
3
2
2
1
1
1
1
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Available images

Catalog No. ABIN2657370
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Top referenced anti-CD25 antibody for ELISA (Detection)

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 41 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 108 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 46 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Rat (Rattus)
(557), (438), (151), (17), (14), (9), (8), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(553), (379), (141), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Alexa Fluor 488
(126), (112), (90), (54), (28), (27), (26), (23), (22), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Detection), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(881), (153), (149), (142), (108), (83), (78), (69), (45), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2657370) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone OX-39
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2657370) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2657370) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2657370) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD25 Antibody (ABIN2657370)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Dignass, Podolsky: "Interleukin 2 modulates intestinal epithelial cell function in vitro." in: Experimental cell research, Vol. 225, Issue 2, pp. 422-9, 1996

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988

Tellides, Dallman, Kupiec-Weglinski, Diamantstein, Morris: "Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4231-3, 1987

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2657370) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (Alexa Fluor 488) (ABIN2657370) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (Alexa Fluor 488)