anti-Rat (Rattus) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Rat (Rattus)
577
453
151
17
15
9
8
6
5
4
3
2
1
1
Host
Mouse
564
393
149
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
116
93
56
29
28
28
23
23
22
17
16
12
11
11
11
11
11
10
9
8
8
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
915
156
155
153
109
84
84
72
48
21
19
17
16
14
13
12
8
7
6
6
6
5
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN120429
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.342041 ABIN255781 FACS IHC (fro) IHC (p) IP IHC ELISA Mouse IgG1 Chain alpha Log in to see OX-39 4
13.342041 ABIN2479468 ELISA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 4
13.342041 ABIN2661002 FACS Biotin Mouse IgG1 kappa Log in to see OX-39 3
13.342041 ABIN2663212 FACS IP PE Mouse IgG1 kappa Log in to see OX-39 3
13.342041 ABIN2657370 ELISA (Detection) FACS IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see OX-39 3
13.342041 ABIN120431 EIA Func FACS IHC (fro) Mouse IgG1 Log in to see NDS 61
13.342041 ABIN2479465 IHC (fro) FACS IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39 4
13.342041 ABIN4896463 FACS PE Mouse IgG1 AA 22-235 Log in to see 745520
13.342041 ABIN4896473 FACS PE Goat IgG AA 22-236 Log in to see Polyclonal
13.342041 ABIN4896474 FACS PE Goat IgG AA 22-236 Log in to see Polyclonal
1 ABIN120427 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see OX-39 3
1 ABIN120430 FACS PE Mouse IgG1 Log in to see OX-39 1
1 ABIN120428 EIA FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN120426 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN320308 FACS FITC Mouse IgG1 Log in to see OX-39 3
1 ABIN120425 FACS FITC Mouse IgG1 Log in to see OX-39 3
1 ABIN120433 EIA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 1
1 ABIN120434 EIA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 3
1 ABIN476836 FACS IHC (fro) APC Mouse IgG1 Log in to see OX-39
1 ABIN2689122 FACS Biotin Mouse IgG1, kappa Log in to see OX 3

Top referenced anti-CD25 antibody for Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 41 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 108 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 46 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Rat (Rattus)
(577), (453), (151), (17), (15), (9), (8), (6), (5), (4), (3), (2), (1), (1)
Host Mouse
(564), (393), (149), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (116), (93), (56), (29), (28), (28), (23), (23), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (9), (8), (8), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(915), (156), (155), (153), (109), (84), (84), (72), (48), (21), (19), (17), (16), (14), (13), (12), (8), (7), (6), (6), (6), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN120429) Images
Specificity This monoclonal antibody recognizes the smaller (alpha subunit) 55 kDa chain of the IL-2 receptor found on activated rat T cells, thymic dendritic cells but not resting lymphocytes. This antibody binds to the rat interleukin-2 receptor designated CD25 and has proven to be an important marker for activated T cells.
Purification Protein G Chromatography
Immunogen T blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens. Donor: BALB/c spleen Fusion Partner: NSO/1
Clone OX-39
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN120429) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 25704
NCBI Accession NP_037295
UniProt P26897
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN120429) Images back to top
Application Notes Flow cytometry. (Reported to be useful in Immunohistochemistry on frozen and paraffin sections.)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN120429) Images back to top
Concentration 1,0 mg/mL
Buffer PBS and 0.02 % NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD25 antibody (ABIN120429)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN120429) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (ABIN120429) Mouse anti CD25 (IL-2 receptor alpha chain) OX-39