anti-Rat (Rattus) CD25 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
Chain alpha
22
21
20
19
18
15
14
14
13
13
7
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Rat (Rattus)
591
472
165
22
19
15
9
8
6
6
4
4
3
3
2
1
Host
Mouse
589
398
151
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
118
95
56
30
29
28
24
23
22
18
16
14
12
12
12
12
12
10
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
931
186
168
155
124
89
85
82
61
29
21
21
19
17
13
12
9
7
6
6
6
5
3
2
1
1
1
1
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Catalog No. ABIN255781
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120427 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see OX-39 3
1 ABIN120429 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see OX-39 3
1 ABIN2479468 ELISA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 4
1 ABIN120428 EIA FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN120433 EIA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 1
1 ABIN120434 EIA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 3
1 ABIN2479465 IHC (fro) FACS IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39 4
1 ABIN120426 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN2689119 BR IHC (fro) FACS IP Mouse IgG1, kappa Log in to see OX 4
1 ABIN476836 FACS IHC (fro) APC Mouse IgG1 Log in to see OX-39
1 ABIN120431 EIA Func FACS IHC (fro) Mouse IgG1 Log in to see NDS 61
1 ABIN4290997 ELISA FACS IHC IHC (fro) IHC (p) IP Mouse IgG1 Chain alpha Log in to see OX-39
1 ABIN2479475 Func IHC (fro) FACS IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39 4
1 ABIN206702 FACS IHC (fro) IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39
1 ABIN4291009 ELISA FACS IHC (fro) IHC (p) DyLight 350 Mouse IgG1 Chain alpha Log in to see OX-39
1 ABIN4291010 ELISA FACS IHC (fro) IHC (p) DyLight 405 Mouse IgG1 Chain alpha Log in to see OX-39
1 ABIN4291011 ELISA FACS IHC IHC (fro) IHC (p) IP PerCP Mouse IgG1 Chain alpha Log in to see OX-39
1 ABIN4291013 ELISA FACS IHC (fro) IHC (p) DyLight 755 Mouse IgG1 Chain alpha Log in to see OX-39
1 ABIN4291014 ELISA FACS IHC (fro) IHC (p) DyLight 680 Mouse IgG1 Chain alpha Log in to see OX-39
1 ABIN4291015 ELISA FACS IHC (fro) IHC (p) DyLight 488 Mouse IgG1 Chain alpha Log in to see OX-39

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope Chain alpha
(22), (21), (20), (19), (18), (15), (14), (14), (13), (13), (7), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(591), (472), (165), (22), (19), (15), (9), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(589), (398), (151), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (118), (95), (56), (30), (29), (28), (24), (23), (22), (18), (16), (14), (12), (12), (12), (12), (12), (10), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
(931), (186), (168), (155), (124), (89), (85), (82), (61), (29), (21), (21), (19), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN255781) Images
Specificity This antibody recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Purification Protein G purified
Immunogen Stimulated Rat T cells
Clone OX-39
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN255781) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Gene Symbol: IL2RA
Gene ID 3559
UniProt P26897
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN255781) Images back to top
Application Notes Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN255781) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-CD25 antibody (ABIN255781)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Crook, Hunt: "Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82." in: Developmental immunology, Vol. 4, Issue 4, pp. 235-46, 1996

Lazo, Klein-Szanto, Tsichlis: "T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications." in: Journal of virology, Vol. 64, Issue 8, pp. 3948-59, 1990 (Sample species: Rat (Rattus)). Further details: Flow Cytometry,Immunohistochemistry (Frozen Sections)

Kampinga, Schuurman, Pol, Bartels, Broekhuizen, Vaessen, Tielen, Rozing, Blaauw, Roser: "Vascular thymus transplantation in rats. Technique, morphology, and function." in: Transplantation, Vol. 50, Issue 4, pp. 669-78, 1990

Bouchot, Anegon, Romaniuk, Jacques, Paineau, Soulillou: "Interleukin 2 receptor in rat heart allograft rejection." in: Transplantation, Vol. 48, Issue 6, pp. 918-22, 1990

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN255781) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN255781) Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [ABIN2557811] - Rat...
Immunofluorescence (IF) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN255781) Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [ABIN25578...
Immunofluorescence (IF) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN255781) Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [ABIN25578...
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN255781) Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [ABIN2557811] - Staining of Con A ...