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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||77 Antibodies|
|Functional Studies (Func)||7 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||6 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||28 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies|
|Immunoprecipitation (IP)||16 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Western Blotting (WB)||20 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|3 references available|
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Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN120427) Images
|Specificity||This monoclonal antibody recognizes the smaller (alpha subunit) 55 kDa chain of the IL-2 receptor found on activated rat T cells, thymic dendritic cells but not resting lymphocytes. This antibody binds to the rat interleukin-2 receptor designated CD25 and has proven to be an important marker for activated T cells (2).|
|Purification||Protein G Chromatography|
|Immunogen||T blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens. Donor: BALB/c spleen Fusion Partner: NSO/1|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN120427) Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN120427) Images back to top
Flow cytometry. Immunohistochemistry on frozen and paraffin sections. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN120427) Images back to top
|Buffer||PBS and 0.02 % NaN3|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD25 Antibody (ABIN120427)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).
Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).
Paterson, Jefferies, Green et al.: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988 (PubMed).
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN120427) back to top