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anti-Rat (Rattus) CD25 antibody for Neutralization

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
AA 116-122, Extracellular Domain
21
19
19
18
15
14
14
7
7
6
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
563
435
150
19
10
9
6
4
3
3
2
1
1
Host
Rabbit
570
383
134
7
5
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
132
121
95
57
27
27
25
23
21
19
17
13
12
10
10
10
10
10
10
10
8
8
8
6
6
6
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
890
151
143
131
113
81
80
74
45
21
21
18
17
12
10
9
7
6
6
5
3
3
3
2
2
1
1
1
Supplier
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Catalog No. ABIN4291070
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008740021 ABIN4291069 BP FACS IF IHC Neut WB Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.0008740021 ABIN2224473 FACS Neut IHC ELISA Mouse IgG1 Log in to see 3H1161

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
ELISA 48 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 114 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 40 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 116-122, Extracellular Domain
(21), (19), (19), (18), (15), (14), (14), (7), (7), (6), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(563), (435), (150), (19), (10), (9), (6), (4), (3), (3), (2), (1), (1)
Host Rabbit
(570), (383), (134), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(132), (121), (95), (57), (27), (27), (25), (23), (21), (19), (17), (13), (12), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Peptide (BP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(890), (151), (143), (131), (113), (81), (80), (74), (45), (21), (21), (18), (17), (12), (10), (9), (7), (6), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ( alpha beta yc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Purification Protein A or G purified
Immunogen CD25
Clone Basiliximab
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Gene Symbol: IL2RA
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Western Blot 1:100 - 1:2000, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Blocking/Neutralizing 50 - 200 Molar excess, Immunofluorescence 1:10 - 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Format Liquid
Buffer PBS
Buffer contains: 0.02 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.