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|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Sheep (Ovine) Alternatives|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS)
|1 reference available|
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Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN181952) Images
|Specificity||This antibody is specific for CD25, also known as Interleukin-2 receptor alpha chain.|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Con A-stimulated ovine lymph node cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN181952) Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||Ovine CD25 is expressed by activated T cells.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN181952) Images back to top
Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN181952) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD25 antibody (ABIN181952)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Verhagen, Brandon, Nash: "Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells." in: Immunology, Vol. 79, Issue 3, pp. 471-8, 1993