anti-Sheep (Ovine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Sheep (Ovine)
591
472
166
22
18
15
9
8
6
6
4
4
3
3
2
1
Host
Mouse
589
398
151
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
118
95
56
30
29
28
24
23
22
18
16
14
12
12
12
12
12
10
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
931
187
168
156
125
90
85
83
61
29
21
21
19
17
13
12
9
7
6
6
6
5
3
2
1
1
1
1
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Catalog No. ABIN181952
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181070 FACS FITC Mouse IgG1 Log in to see IL-A111 2
1 ABIN2479442 IHC (fro) FACS IP Mouse IgG1 Log in to see IL-A111 4
1 ABIN2479443 FACS FITC Mouse IgG1 Log in to see IL-A111 4
1 ABIN596065 FACS PE Mouse IgG1 Log in to see
1 ABIN316523 FACS PE Mouse IgG1 Log in to see IL-A111
1 ABIN2479444 FACS PE Mouse IgG1 Log in to see IL-A111 4
1 ABIN2479478 FACS FITC Mouse IgG1 Log in to see 9-14 3
1 ABIN596064 FACS FITC Mouse IgG1 Log in to see
1 ABIN601651 IHC (fro) FACS IP Mouse IgG1 Log in to see
1 ABIN5625393 FACS Func IHC Mouse IgG1 Log in to see CACT116A
1 ABIN5625395 FACS Func IHC Mouse IgG1 Log in to see CACT116A
1 ABIN5625398 FACS Func IHC Mouse IgG1 Log in to see GB112A
1 ABIN5625401 FACS Func IHC Mouse IgG3 Log in to see LCTB2A
1 ABIN2224526 FACS FITC Mouse IgG1 Log in to see 6A262
1 ABIN576883 FACS IHC (fro) IP PE Mouse IgG1 Log in to see IL-A111
1 ABIN206375 FACS IHC (fro) IP FITC Mouse IgG1 Log in to see
1 ABIN5559742 FACS IHC (fro) IP Mouse IgG1 Log in to see 9-14
1 ABIN206834 FACS IHC (fro) IP FITC Mouse IgG1 Log in to see

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Sheep (Ovine)
(591), (472), (166), (22), (18), (15), (9), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(589), (398), (151), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (118), (95), (56), (30), (29), (28), (24), (23), (22), (18), (16), (14), (12), (12), (12), (12), (12), (10), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(931), (187), (168), (156), (125), (90), (85), (83), (61), (29), (21), (21), (19), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN181952) Images
Specificity This antibody is specific for CD25, also known as Interleukin-2 receptor alpha chain.
Purification Affinity chromatography on Protein G
Immunogen Con A-stimulated ovine lymph node cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Clone 01-09-14
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling ProductDetails: References for anti-CD25 antibody (ABIN181952) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background Ovine CD25 is expressed by activated T cells.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 443435
NCBI Accession NP_001009415
UniProt P26898
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling ProductDetails: References for anti-CD25 antibody (ABIN181952) Images back to top
Application Notes Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details ProductDetails: References for anti-CD25 antibody (ABIN181952) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD25 antibody (ABIN181952)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Verhagen, Brandon, Nash: "Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells." in: Immunology, Vol. 79, Issue 3, pp. 471-8, 1993