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anti-Sheep (Ovine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Sheep (Ovine)
569
457
169
19
10
9
8
5
4
3
3
2
1
1
Host
Mouse
601
406
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
124
101
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
936
145
141
129
127
121
80
72
48
21
18
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN181952
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010616893 ABIN181070 FACS FITC Mouse IgG1 Log in to see IL-A111 2
0.0010616893 ABIN256223 FACS IHC (fro) IP IHC Mouse IgG1 Log in to see 9-14
0.0010616893 ABIN316523 FACS PE Mouse IgG1 Log in to see IL-A111
0.0010616893 ABIN206375 FACS FITC Mouse IgG1 Log in to see
0.0010616893 ABIN2224526 FACS FITC Mouse IgG1 Log in to see 6A262
0.0010616893 ABIN2479443 FACS FITC Mouse IgG1 Log in to see IL-A111 4
0.0010616893 ABIN2479444 FACS PE Mouse IgG1 Log in to see IL-A111 4
0.0010616893 ABIN2479478 FACS FITC Mouse IgG1 Log in to see 9-14 3
0.0010616893 ABIN2479442 IHC (fro) FACS IP Mouse IgG1 Log in to see IL-A111 4
0.0010616893 ABIN2490281 FACS FITC Mouse IgG1 Log in to see

Top referenced anti-CD25 antibody for Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Sheep (Ovine)
Flow Cytometry (FACS) 11 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Sheep (Ovine)
(569), (457), (169), (19), (10), (9), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(601), (406), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (124), (101), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(936), (145), (141), (129), (127), (121), (80), (72), (48), (21), (18), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN181952) Images
Specificity This antibody is specific for CD25, also known as Interleukin-2 receptor alpha chain.
Purification Affinity chromatography on Protein G
Immunogen Con A-stimulated ovine lymph node cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Clone 01-09-14
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN181952) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background Ovine CD25 is expressed by activated T cells.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 443435
NCBI Accession NP_001009415
UniProt P26898
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN181952) Images back to top
Application Notes Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN181952) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD25 Antibody (ABIN181952)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Verhagen, Brandon, Nash: "Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells." in: Immunology, Vol. 79, Issue 3, pp. 471-8, 1993