You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Sheep (Ovine) CD25 antibody for Immunohistochemistry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Sheep (Ovine)
577
462
170
19
10
10
8
5
4
3
3
2
1
1
Host
Mouse
609
411
135
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
125
102
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (IHC)
948
149
142
132
130
125
84
74
48
21
20
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN256223
Contact our Customer Service for availability and price in your country.

Similar anti-CD25 Antibodies

Application / Reactivity Sheep (Ovine)
Flow Cytometry (FACS) 11 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Sheep (Ovine)
(577), (462), (170), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(609), (411), (135), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (125), (102), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(948), (149), (142), (132), (130), (125), (84), (74), (48), (21), (20), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity Specific for ovine CD25, also known as Interleukin-2 receptor alpha chain. Ovine CD25 is expressed by activated T cells. This immunoprecipitates a band of approximately 47kD under reducing conditions.
Purification Protein G purified
Immunogen Con A-stimulated ovine lymph node cells
Clone 9-14
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The IL2 receptor (IL2R) exists in three alternative forms made up from the individualcomponents of CD25, CD122, and CD132. CD25 represents the low affinity alpha chainof the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is richin O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed onactivated T cells, B cells, NK cells and monocytes of all mouse strains tested. It istransiently expressed at a low level during B cell development in bone marrow on theCD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature Bcells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor onCD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher levelduring very early T cell development in fetal and adult thymus. Alternate Names: anti-CD 25 antibody, anti-CD25 antibody, anti-CD25 antigen antibody, anti-IDDM10antibody, anti-IL 2 RA antibody, anti-IL 2 receptor alpha subunit antibody, anti-IL 2Rantibody, anti-IL2 RA antibody, anti-IL2R antibody, anti-IL2RA antibody, anti-IL2RACantibody, anti-Interleukin 2 Receptor Alpha antibody, anti-Interleukin 2 receptor alphachain antibody, anti-Ly43 antibody, anti-p55 antibody, anti-T Cell Growth FactorReceptor antibody, anti-TAC Antigen antibody, anti-TCGFR antibody.
Gene Symbol: IL2RA
Gene ID 3559
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:10-1:50, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Immunoprecipitation 1:10-1:500
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline pH 7.4, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.