Mouse (Murine) CD25 ELISA Kit

Recommended CD25 Product (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) ELISA Kits
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
AA 22-236
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
42
13
12
5
3
3
2
1
1
Method Type
Sandwich ELISA
Detection Range
78-5000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
Options
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Catalog No. ABIN1672833
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.704079 ABIN1029825 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
9.704079 ABIN425137 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
9.704079 ABIN366354 Sandwich ELISA 78-5000 pg/mL 78 pg/mL Log in to see
9.704079 ABIN625409 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 10-2500 pg/mL 10 pg/mL Log in to see
9.704079 ABIN2708455 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN5563446 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 10.2-2500 pg/mL 10.2 pg/mL Log in to see
1 ABIN2042394 Sandwich ELISA 8-5000 pg/mL 8 pg/mL Log in to see
1 ABIN2684684 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2535485 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
1 ABIN4970030 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2948878 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN455618 Log in to see

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General

Antigen Interleukin 2 Receptor, alpha (IL2RA) ELISA Kits
Epitope AA 22-236
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(42), (13), (12), (5), (3), (3), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 78-5000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CD25 ELISA Kit

Target details Application Details Handling ProductDetails: References for CD25 Kit (ABIN1672833) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD25/IL-2sR alpha
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO,E22-K236
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E22-K236

Target details

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Antigen
Alternative Name IL2RA (IL2RA ELISA Kit Abstract)
Background

Protein Function: Receptor for interleukin-2.

Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Synonyms: Interleukin-2 receptor subunit alpha,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,p55,CD25,Il2ra,Il2r,

Full Gene Name: Interleukin-2 receptor subunit alpha

Cellular Localisation: Membrane, Single-pass type I membrane protein.
Gene ID 16184
UniProt P01590
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated
Protocol mouse CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-K236) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD25 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CD25 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 564, Standard deviation: 32.7, CV(%): 5.8
  • Sample 2: n=16, Mean(pg/ml): 1244, Standard deviation: 75.9, CV(%): 6.1
  • Sample 3: n=16, Mean(pg/ml): 2860, Standard deviation: 157.3, CV(%): 5.5,
  • Sample 1: n=24, Mean(pg/ml): 758, Standard deviation: 54.6, CV(%): 7.2
  • Sample 2: n=24, Mean(pg/ml): 1654, Standard deviation: 134, CV(%): 8.1
  • Sample 3: n=24, Mean(pg/ml): 3242, Standard deviation: 217, CV(%): 6.7
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for CD25 Kit (ABIN1672833)

Product Details CD25 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Webb, Campbell, Lee, Young: "Mapping the gene for murine T-cell growth factor, Il-2, to bands B-C on chromosome 3 and for the alpha chain of the IL2-receptor, Il-2ra, to bands A2-A3 on chromosome 2." in: Cytogenetics and cell genetics, Vol. 54, Issue 3-4, pp. 164-8, 1991

Leonard, Depper, Kanehisa, Krönke, Peffer, Svetlik, Sullivan, Greene: "Structure of the human interleukin-2 receptor gene." in: Science (New York, N.Y.), Vol. 230, Issue 4726, pp. 633-9, 1985

Images

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Supplier Images
ELISA image for CD25 ELISA Kit (Interleukin 2 Receptor, alpha) (ABIN1672833) Mouse CD25/IL-2sR alpha PicoKine ELISA Kit standard curve