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anti-Human CNTF antibody for Immunohistochemistry (Frozen Sections)

Recommended CNTF Antibody (supplied by: Log in to see )

Antigen
Ciliary Neurotrophic Factor (CNTF) Antibodies
  • AI429687
  • CNTF
  • GPA
  • HCNTF
Reactivity
Human
153
73
38
3
2
1
1
Host
Rabbit
179
16
8
3
3
Clonality
Polyclonal
Conjugate
This CNTF antibody is un-conjugated
34
14
9
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
155
127
34
23
12
11
11
9
7
6
5
5
3
3
1
1
1
1
Supplier
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Catalog No. ABIN116302
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008842227 ABIN116301 EIA Func IHC (fro) WB Rabbit Log in to see Polyclonal
0.0008842227 ABIN1171995 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-CNTF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 86 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Functional Studies (Func) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Immunostaining (ISt) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 111 Antibodies

General

Antigen Ciliary Neurotrophic Factor (CNTF) Antibodies
Reactivity Human
(153), (73), (38), (3), (2), (1), (1)
Host Rabbit
(179), (16), (8), (3), (3)
Clonality
Polyclonal
Conjugate This CNTF antibody is un-conjugated
(34), (14), (9), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(155), (127), (34), (23), (12), (11), (11), (9), (7), (6), (5), (5), (3), (3), (1), (1), (1), (1)
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Product Details anti-CNTF Antibody

Target Details CNTF Application Details Handling Images
Specificity Ciliary Neurotrophic Factor (CNTF)
Purification Affinity chromatography.
Immunogen Highly pure (>98%) recombinant hCNTF.

Target Details CNTF

Product Details anti-CNTF Antibody Application Details Handling Images back to top
Antigen
Alternative Name CNTF (CNTF Antibody Abstract)
Background Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.Synonyms: Ciliary Neurotrophic Factor
Gene ID 1270
NCBI Accession NP_000605
UniProt P26441
Research Area Stem Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CNTF Antibody Target Details CNTF Handling Images back to top
Application Notes Neutralization. ELISA: To detect hCNTF by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hCNTF. Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hCNTF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CNTF Antibody Target Details CNTF Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C or in aliquots at -20 °C long term.