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anti-Rat (Rattus) CNTF antibody for Immunocytochemistry

Recommended CNTF Antibody (supplied by: Log in to see )

Antigen
Ciliary Neurotrophic Factor (CNTF) Antibodies
  • AI429687
  • CNTF
  • GPA
  • HCNTF
Epitope
AA 2-200
15
13
12
10
9
4
3
3
2
1
1
1
1
1
1
Reactivity
Rat (Rattus)
158
74
40
3
2
1
1
1
Host
Rabbit
180
17
10
3
3
Clonality
Polyclonal
Conjugate
This CNTF antibody is un-conjugated
34
14
9
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
159
127
36
23
13
12
11
9
8
6
5
5
4
3
1
1
1
1
1
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Catalog No. ABIN1077934
$ 376.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010086668 ABIN1171999 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal

Top referenced anti-CNTF antibody for Immunocytochemistry

Similar anti-CNTF Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
ELISA 41 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 11 Antibodies
Western Blotting (WB) 58 Antibodies

General

Antigen Ciliary Neurotrophic Factor (CNTF) Antibodies
Epitope AA 2-200
(15), (13), (12), (10), (9), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(158), (74), (40), (3), (2), (1), (1), (1)
Host Rabbit
(180), (17), (10), (3), (3)
Clonality
Polyclonal
Conjugate This CNTF antibody is un-conjugated
(34), (14), (9), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(159), (127), (36), (23), (13), (12), (11), (9), (8), (6), (5), (5), (4), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CNTF Antibody

Target Details CNTF Application Details Handling References for anti-CNTF Antibody (ABIN1077934) Images
Sequence MGSSHHHH HHSSGLVPRG SHMASMTGGQ QMGRGS-AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
Specificity It has been selected for its ability to recognize CNTF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant CNTF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CNTF.
Isotype IgG

Target Details CNTF

Product Details anti-CNTF Antibody Application Details Handling References for anti-CNTF Antibody (ABIN1077934) Images back to top
Antigen
Research Area Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CNTF Antibody Target Details CNTF Handling References for anti-CNTF Antibody (ABIN1077934) Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant CNTF (Ala2~Met200) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-CNTF Antibody Target Details CNTF Application Details References for anti-CNTF Antibody (ABIN1077934) Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

References for anti-CNTF Antibody (ABIN1077934)

Product Details anti-CNTF Antibody Target Details CNTF Application Details Handling Images back to top
Product cited in:

Liu, Xu, Yang, Chen, Liu, Li, Li: "Analysis of olfactory ensheathing glia transplantation-induced repair of spinal cord injury by electrophysiological, behavioral, and histochemical methods in rats." in: Journal of molecular neuroscience : MN, Vol. 41, Issue 1, pp. 25-9, 2010

Images

Product Details anti-CNTF Antibody Target Details CNTF Application Details Handling References for anti-CNTF Antibody (ABIN1077934) back to top
Supplier Images
Western Blotting (WB) image for anti-CNTF antibody (Ciliary Neurotrophic Factor) (AA 2-200) (ABIN1077934) anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibody