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Rat (Rattus) CNTF ELISA Kit

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Antigen
Ciliary Neurotrophic Factor (CNTF) ELISA Kits
  • AI429687
  • CNTF
  • GPA
  • HCNTF
Epitope
AA 2-200
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
17
17
12
10
10
9
3
3
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1672723
$ 333.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN416073 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 39.06-2500 pg/mL 39.06 pg/mL Log in to see
0.32666028 ABIN1114156 Sandwich ELISA Serum, Plasma, Biological Fluids 39.06-2500 pg/mL 39.06 pg/mL Log in to see
0.32666028 ABIN2708633 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012121415 ABIN366460 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
0.0012121415 ABIN625191 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 10-2000 pg/mL 10 pg/mL Log in to see 3
0.0012121415 ABIN626896 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.0012121415 ABIN1569059 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 39.063-2500 pg/mL 39.063 pg/mL Log in to see
0.0012121415 ABIN512582 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012121415 ABIN2683843 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Log in to see 1
0.0012121415 ABIN2042409 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012121415 ABIN2115919 10-4000 μIU/mL 10 μIU/mL Log in to see
0.0012121415 ABIN579288 78-5000 pg/mL 78 pg/mL Log in to see
0.0012121415 ABIN2542978 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 39.06-2500 pg/mL 39.06 pg/mL Log in to see
0.0012121415 ABIN2073974 Sandwich ELISA Log in to see
0.0012121415 ABIN2946349 39.063-2500 pg/mL 39.063 pg/mL Log in to see

Top referenced CNTF ELISA Kit for ELISA

Similar CNTF ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 16 ELISA Kits

General

Antigen Ciliary Neurotrophic Factor (CNTF) ELISA Kits
Epitope AA 2-200
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(17), (17), (12), (10), (10), (9), (3), (3), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CNTF ELISA Kit

Target details Application Details Handling References for CNTF Kit (ABIN1672723) Images
Purpose For quantitative detection of rat CNTF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant rat CNTF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat CNTF antibod - 1
  • Lyophilized recombinant rat CNTF standar - 10ng/tubex2
  • Biotinylated anti- rat CNTF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A2-M200

Target details

Product Details CNTF ELISA Kit Application Details Handling References for CNTF Kit (ABIN1672723) Images back to top
Antigen
Alternative Name CNTF (CNTF ELISA Kit Abstract)
Background Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85 % homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
Gene ID 25707
UniProt P20294
Research Area Stem Cells
Pathways JAK-STAT Signaling

Application Details

Product Details CNTF ELISA Kit Target details Handling References for CNTF Kit (ABIN1672723) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat CNTF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CNTF has been precoated onto 96-well plates. Standards(E.coli, A2-M200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CNTF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CNTF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat CNTF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat CNTF standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 107, Standard deviation: 4.8, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 452, Standard deviation: 18.9, CV(%): 4.2
  • Sample 3: n=16, Mean(pg/ml): 1287, Standard deviation: 50.2, CV(%): 3.9,
  • Sample 1: n=24, Mean(pg/ml): 193, Standard deviation: 16.2, CV(%): 8.4
  • Sample 2: n=24, Mean(pg/ml): 643, Standard deviation: 52.7, CV(%): 8.2
  • Sample 3: n=24, Mean(pg/ml): 1057, Standard deviation: 77.2, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details CNTF ELISA Kit Target details Application Details References for CNTF Kit (ABIN1672723) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CNTF Kit (ABIN1672723)

Product Details CNTF ELISA Kit Target details Application Details Handling Images back to top
Background publications

Watt, Dzamko, Thomas, Rose-John, Ernst, Carling, Kemp, Febbraio, Steinberg: "CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK." in: Nature medicine, Vol. 12, Issue 5, pp. 541-8, 2006

Lam, Fuller, Miller, Kloss, Manthorpe, Varon, Cordell: "Sequence and structural organization of the human gene encoding ciliary neurotrophic factor." in: Gene, Vol. 102, Issue 2, pp. 271-6, 1991

Images

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Supplier Images
ELISA image for CNTF ELISA Kit (Ciliary Neurotrophic Factor) (ABIN1672723) Rat CNTF PicoKine ELISA Kit standard curve