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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|Enzyme Immunoassay (EIA)||24 Antibodies|
|Flow Cytometry (FACS)||102 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunoassay (IA)||3 Antibodies|
|Immunocytochemistry (ICC)||51 Antibodies|
|Immunodiffusion (ID)||1 Antibodies|
|Immunofluorescence (IF)||67 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||45 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||15 Antibodies|
|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Conjugate||This CSF2 antibody is conjugated to Biotin Alternatives|
ELISA (Detection), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN371617) Images
|Specificity||This antibody is specific to Human Granulocyte/Macrophage-Colony Stimulating Factor (GM-CSF)1-4. The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a Neutralizing antibody).|
|Characteristics||Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2|
|Immunogen||E. coli-expressed human GM-CSF.|
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 Antibody (ABIN371617) Images back to top
|Alternative Name||GM-CSF (CSF2 Antibody Abstract)|
|Background||GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim|
|Research Area||Immunology, Innate Immunity, Cytokines|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 Antibody (ABIN371617) Images back to top
ELISA Detection: Biotinylated Monoclonal antibody ABIN371617 is the preferreddetection antibody in a Sandwich ELISA for quantifying human GM-CSF protein levels. (Ref.1-4). It should be paired with purified withpurified recombinant human GM-CSF as the standard. Immunofluorescence/Flow Cytometry: This antibody is useful for Intracytoplasmic stainingand Flow Cytometric analysis to identify and enumerate GM-CSF-positive cells within mixedcell populations. Recombinant human GM-CSF (Cat#ABIN371618 should be used as a Specificity Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 Antibody (ABIN371617) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CSF2 Antibody (ABIN371617)Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992
Kita, Ohnishi, Okubo, Weiler, Abrams, Gleich: "Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 745-8, 1991
Bacchetta, de Waal Malefijt, Yssel, Abrams, de Vries, Spits, Roncarolo: "Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 902-8, 1990