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anti-Human CSF2 antibody for ELISA (Detection)

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • CSF
  • CSF2
  • Csfgm
  • Gm-csf
  • Gm-CSf
  • GM-CSF
  • GMCSF
  • Gmcsf
  • MGI-IGM
Reactivity
Human
370
119
44
27
14
10
4
4
2
2
Host
Rat
196
177
137
10
3
Clonality
Monoclonal
Conjugate
This CSF2 antibody is conjugated to Biotin
58
16
13
13
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA (Detection), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF)
340
279
123
80
76
68
60
53
34
24
20
18
12
7
6
6
6
3
3
3
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN371617
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000875046 ABIN371618 ELISA (Detection) EIA FACS IF Rat IgG2a Log in to see BVD2-21C11 3
0.000875046 ABIN453897 ELISA (Detection) WB Mouse IgG1 Log in to see 429

Top referenced anti-CSF2 antibody for ELISA (Detection)

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General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Reactivity Human
(370), (119), (44), (27), (14), (10), (4), (4), (2), (2)
Host Rat
(196), (177), (137), (10), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2 antibody is conjugated to Biotin
(58), (16), (13), (13), (7), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application ELISA (Detection), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF)
(340), (279), (123), (80), (76), (68), (60), (53), (34), (24), (20), (18), (12), (7), (6), (6), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN371617) Images
Specificity This antibody is specific to Human Granulocyte/Macrophage-Colony Stimulating Factor (GM-CSF)1-4. The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a Neutralizing antibody).
Characteristics Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2
Immunogen E. coli-expressed human GM-CSF.
Clone BVD2-21C11
Isotype IgG2a

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 Antibody (ABIN371617) Images back to top
Antigen
Alternative Name GM-CSF (CSF2 Antibody Abstract)
Background GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim
Gene ID 1437
UniProt P04141
Research Area Immunology, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 Antibody (ABIN371617) Images back to top
Application Notes ELISA Detection: Biotinylated Monoclonal antibody ABIN371617 is the preferreddetection antibody in a Sandwich ELISA for quantifying human GM-CSF protein levels. (Ref.1-4). It should be paired with purified withpurified recombinant human GM-CSF as the standard. Immunofluorescence/Flow Cytometry: This antibody is useful for Intracytoplasmic stainingand Flow Cytometric analysis to identify and enumerate GM-CSF-positive cells within mixedcell populations. Recombinant human GM-CSF (Cat#ABIN371618 should be used as a Specificity Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 Antibody (ABIN371617) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CSF2 Antibody (ABIN371617)

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
Background publications

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Kita, Ohnishi, Okubo, Weiler, Abrams, Gleich: "Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 745-8, 1991

Bacchetta, de Waal Malefijt, Yssel, Abrams, de Vries, Spits, Roncarolo: "Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 902-8, 1990