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anti-Human CSF2 antibody for Immunohistochemistry (Frozen Sections)

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • CSF
  • CSF2
  • Csfgm
  • Gm-csf
  • Gm-CSf
  • GM-CSF
  • GMCSF
  • Gmcsf
  • MGI-IGM
Reactivity
Human
370
119
44
27
14
10
4
4
2
2
Host
Mouse
196
178
136
10
3
Clonality
Monoclonal
Conjugate
This CSF2 antibody is un-conjugated
59
16
13
13
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
339
279
124
80
76
69
60
53
34
23
19
18
12
7
6
6
6
4
3
3
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN180855
$ 577.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000877444 ABIN181022 EIA IHC (fro) Rat IgG2a Log in to see BVD2-23B6
0.000877444 ABIN1859034 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 18-144 Log in to see Polyclonal
0.000877444 ABIN205653 Func IHC (fro) ELISA WB Mouse IgG1 Log in to see
0.000877444 ABIN601431 Func IHC (fro) ELISA WB Mouse IgG1 Log in to see
0.000877444 ABIN255988 Func IHC (fro) IHC ELISA WB Mouse IgG1 Log in to see 30-4
0.000877444 ABIN116866 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
0.000877444 ABIN4315141 BP ELISA FACS ICC IF IHC (fro) IP Neut WB Rat IgG2a kappa Log in to see BVD2-21C11
0.000877444 ABIN4315142 BP ELISA FACS ICC IF IHC (fro) IP Neut WB Rat IgG2a kappa Log in to see BVD2-21C11
0.000877444 ABIN4315143 BP ELISA FACS ICC IF IHC (fro) IP Neut WB Rat IgG2a kappa Log in to see SPM469
0.000877444 ABIN4315144 BP ELISA FACS ICC IF IHC (fro) IP Neut WB Rat IgG2a kappa Log in to see SPM469
0.000877444 ABIN4315118 BP IHC IHC (fro) Neut WB Mouse IgG1 Log in to see MM0315-7U1
0.000877444 ABIN3024892 IHC (fro) FACS IF IP Neut WB Rat IgG2a, kappa Log in to see SPM469
0.000877444 ABIN2473849 Func IHC (fro) ELISA WB Mouse IgG1 Log in to see 3209 (30-4)
0.000877444 ABIN3024893 IHC (fro) FACS IF IP Neut WB Rat IgG2a, kappa Log in to see SPM469

Similar anti-CSF2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 189 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 24 Antibodies
Flow Cytometry (FACS) 102 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 51 Antibodies
Immunodiffusion (ID) 1 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Reactivity Human
(370), (119), (44), (27), (14), (10), (4), (4), (2), (2)
Host Mouse
(196), (178), (136), (10), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2 antibody is un-conjugated
(59), (16), (13), (13), (7), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(339), (279), (124), (80), (76), (69), (60), (53), (34), (23), (19), (18), (12), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling Images
Specificity When immobilised on a microplate, this antibody will capture recombinant and natural GM-CSF.
Characteristics Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2
Purification Protein A chromatography, 0.2µm sterile filtered
Immunogen R-DNA. Genename: GM-CSF
Clone 30-04-14
Isotype IgG1

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GM-CSF (CSF2 Antibody Abstract)
Background The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim
Gene ID 1437
UniProt P04141
Research Area Immunology, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
Application Notes Immunohistochemistry on frozen sections: used at 25g/mLELISA: 1/1K - 1/2K. Western Blot: used at 1-2g/mLFunctional Assays: The neutralisation dose (ND50) of this batch was determined to beapproximately 0.3-0.5 μg/mL in the presence of 0.5 ng/mL of recombinant human GM-CSF. ND50 may vary depending on conditions. ND50 is defined as the concentration of Abrequired to yield half maximal inhibition of cytokine activity on a responsive cell line, whenthat cytokine is present at a concentration just high enough to elicit a maximum response.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 with 1 % Trehalose
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months