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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|Enzyme Immunoassay (EIA)||24 Antibodies|
|Flow Cytometry (FACS)||102 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunoassay (IA)||3 Antibodies|
|Immunocytochemistry (ICC)||51 Antibodies|
|Immunodiffusion (ID)||1 Antibodies|
|Immunofluorescence (IF)||67 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||45 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||15 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies|
|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Conjugate||This CSF2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling Images
|Specificity||When immobilised on a microplate, this antibody will capture recombinant and natural GM-CSF.|
|Characteristics||Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2|
|Purification||Protein A chromatography, 0.2µm sterile filtered|
|Immunogen||R-DNA. Genename: GM-CSF|
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling Images back to top
|Alternative Name||GM-CSF (CSF2 Antibody Abstract)|
|Background||The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim|
|Research Area||Immunology, Innate Immunity, Cytokines|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
Immunohistochemistry on frozen sections: used at 25g/mLELISA: 1/1K - 1/2K. Western Blot: used at 1-2g/mLFunctional Assays: The neutralisation dose (ND50) of this batch was determined to beapproximately 0.3-0.5 μg/mL in the presence of 0.5 ng/mL of recombinant human GM-CSF. ND50 may vary depending on conditions. ND50 is defined as the concentration of Abrequired to yield half maximal inhibition of cytokine activity on a responsive cell line, whenthat cytokine is present at a concentration just high enough to elicit a maximum response.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details Images back to top
|Buffer||PBS, pH 7.2 with 1 % Trehalose|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|