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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||3 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies|
|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CSF2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Neutralization (Neut), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN401109) Images
|Specificity||This antibody reacts to Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF).|
|Immunogen||This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.|
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 Antibody (ABIN401109) Images back to top
|Alternative Name||GM-CSF (CSF2 Antibody Abstract)|
|Background||Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim|
|Research Area||Immunology, Innate Immunity, Cytokines|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 Antibody (ABIN401109) Images back to top
ELISA: 1/20000 - 1/100000. Western Blot: 1/500 - 1/2000. Neutralization: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 Antibody (ABIN401109) Images back to top
|Reconstitution||Restore with 0.1 mL of deionized water (or equivalent).|
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CSF2 Antibody (ABIN401109)Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
DeLamarter, Mermod, Liang et al.: "Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum." in: The EMBO journal, Vol. 4, Issue 10, pp. 2575-81, 1985 (PubMed).
Stanley, Metcalf, Sobieszczuk et al.: "The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters." in: The EMBO journal, Vol. 4, Issue 10, pp. 2569-73, 1985 (PubMed).
Miyatake, Otsuka, Yokota et al.: "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2561-8, 1985 (PubMed).
ImagesProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN401109) back to top