This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Purified, heated to 56°C for 30 minutes
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.