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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||3 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CSF2 antibody is un-conjugated Alternatives|
Neutralization (Neut), ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling Images
|Characteristics||Polyclonal antibody to detect GM-CSF in mouse samples|
|Immunogen||E.coli derived Recombinant Murine GM-CSF|
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling Images back to top
|Alternative Name||GM-CSF (CSF2 Antibody Abstract)|
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, MGC131935
|Research Area||Immunology, Innate Immunity, Cytokines|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
WB: Use 0.1-0.2 μg/mL. The detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA: Use 0.5 - 2.0 μg/mL (100 μL/well antibody solution)
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/mL), a concentration of 0.012-0.020 μg/mL of this antibody is required
Suggested positive control: ELISA: Recombinant mGM-CSF (Cat # 4101)
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details Images back to top
|Buffer||A sterile filtered antibody solution in PBS, pH 7.2.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Expiry Date||12 months|
ImagesProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling back to top