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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||3 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies|
|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CSF2 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (IHC)
|10 references available|
|Supplier||Log in to see|
Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN2665927) Images
|Purification||The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.|
|Sterility||0.2 μm filtered|
Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg of the protein) as determined by the LAL test.
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 Antibody (ABIN2665927) Images back to top
|Alternative Name||GM-CSF (CSF2 Antibody Abstract)|
|Background||GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.|
|Research Area||Immunology, Innate Immunity, Cytokines|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 Antibody (ABIN2665927) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 Antibody (ABIN2665927) Images back to top
|Handling Advice||This LEAF™ solution contains no preservative, handle under aseptic conditions.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CSF2 Antibody (ABIN2665927)Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008 (PubMed).
Larkin, Renukaradhya, Sriram et al.: "CD44 differentially activates mouse NK T cells and conventional T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 268-79, 2006 (PubMed).
Sander, Höidén, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).
Suda, OGarra, MacNeil et al.: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, 1990 (PubMed).
Nozaki, Abrams, Pearce et al.: "Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes." in: The Journal of investigative dermatology, Vol. 97, Issue 1, pp. 10-4, 1991 (PubMed).
Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, 1991 (PubMed).
Fan, OBrian, Ioannides et al.: "Granulocyte-macrophage colony-stimulating factor (GM-CSF) in the management of cancer." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 571-7, 1992 (PubMed).
Demetri, Griffin: "Granulocyte colony-stimulating factor and its receptor." in: Blood, Vol. 78, Issue 11, pp. 2791-808, 1991 (PubMed).
Negrin, Greenberg: "Therapy of hematopoietic disorders with recombinant colony-stimulating factors." in: Advances in pharmacology (San Diego, Calif.), Vol. 23, pp. 263-96, 1992 (PubMed).
Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).
ImagesProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN2665927) back to top