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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies|
|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Epitope||AA 18-141 Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CSF2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Granulocyte-macrophage colony-stimulating factor(CSF2) detection. Tested with WB, IHC-P in Mouse.
Gene Name: colony stimulating factor 2 (granulocyte-macrophage)
Protein Name: Granulocyte-macrophage colony-stimulating factor
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.|
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling Images back to top
|Alternative Name||CSF2 (CSF2 Antibody Abstract)|
|Background||GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.|
|Pathways||JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin|
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for GM-CSF is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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