anti-Rat (Rattus) CSF2 antibody for Immunocytochemistry

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • CSF2
  • GM-CSF
  • GMCSF
  • CSF
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • granulocyte-macrophage colony-stimulating-factor
  • colony stimulating factor 2 (granulocyte-macrophage)
  • granulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • GM-CSF
  • CSF2
  • Csf2
Epitope
AA 1-127
46
13
11
11
10
8
7
5
4
4
3
3
2
1
1
1
1
Reactivity
Rat (Rattus)
228
54
25
7
6
3
3
3
1
1
Host
Mouse
132
113
56
4
2
Clonality
Monoclonal
Conjugate
This CSF2 antibody is un-conjugated
26
10
7
5
4
4
4
4
4
4
4
3
3
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
209
189
63
44
40
25
25
11
10
7
7
5
5
4
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4898951
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5646341 CyTOF FACS ICC Neut WB Mouse IgG2b AA 1-127 Log in to see 83308
1 ABIN1868194 ICC IHC IP WB Rabbit IgG AA 18-144 Log in to see Polyclonal

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Epitope AA 1-127
(46), (13), (11), (11), (10), (8), (7), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(228), (54), (25), (7), (6), (3), (3), (3), (1), (1)
Host Mouse
(132), (113), (56), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2 antibody is un-conjugated
(26), (10), (7), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(209), (189), (63), (44), (40), (25), (25), (11), (10), (7), (7), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling References for anti-CSF2 antibody (ABIN4898951) Images
Specificity Detects rat GM-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant GM-CSF from mouse, human, or pig is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen E. coli-derived recombinant rat GM-CSF, Ala1-Lys127, Accession # P48750
Clone 83308
Isotype IgG2b

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 antibody (ABIN4898951) Images back to top
Antigen
Alternative Name CSF2 (CSF2 Antibody Abstract)
Background Alternative Names: colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF2, GMCSF, GM-CSF, GMCSFgranulocyte-macrophage colony-stimulating factor, granulocyte-macrophage colony stimulating factor, MGC131935, MGC138897, molgramostin, sargramostim
Gene ID 1437
UniProt P48750
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 antibody (ABIN4898951) Images back to top
Application Notes Western Blot 1 μg/mL
Neutralization 1-4 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 antibody (ABIN4898951) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CSF2 antibody (ABIN4898951)

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
Product cited in:

Stojić-Vukanić, Nacka-Aleksić, Pilipović, Vujnović, Blagojević, Kosec, Dimitrijević, Leposavić: "Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats." in: Immunity & ageing : I & A, Vol. 12, pp. 16, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Rat (Rattus)).

Sousa, Halper, Zhang, Lewis, Li: "Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats." in: BMC complementary and alternative medicine, Vol. 8, pp. 5, 2008 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Images

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling References for anti-CSF2 antibody (ABIN4898951) back to top
Supplier Images
Neutralization (Neut) image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-127) antibody (ABIN4898951) Cell Proliferation Induced by GM-CSF and Neutralization by Rat GM-CSF Antibody. Recom...
Immunocytochemistry (ICC) image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-127) antibody (ABIN4898951) GM-CSF in Rat Splenocytes. GM-CSF was detected in immersion fixed rat splenocytes usi...
Immunocytochemistry (ICC) image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-127) antibody (ABIN4898951) GM-CSF in Rat Splenocytes. GM-CSF was detected in immersion fixed rat splenocytes usi...
Neutralization (Neut) image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-127) antibody (ABIN4898951) Cell Proliferation Induced by GM-CSF and Neutralization by Rat GM-CSF Antibody. Recom...