You are viewing an incomplete version of our website. Please click to reload the website as full version.

Human CSF2 ELISA Kit

Recommended CSF2 Product (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kits
  • CSF
  • CSF2
  • Csfgm
  • Gm-csf
  • Gm-CSf
  • GM-CSF
  • GMCSF
  • Gmcsf
  • MGI-IGM
Epitope
AA 18-144
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
47
45
34
11
9
7
5
5
4
4
3
2
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1672729
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN414381 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see 1
0.32666028 ABIN2472069 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32666028 ABIN1379943 Sandwich ELISA Cell Lysate, Serum, Plasma 32-1000 pg/mL 32 pg/mL Log in to see
0.32666028 ABIN1115248 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32666028 ABIN1888512 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 2-500 pg/mL 2 pg/mL Log in to see
0.32666028 ABIN2708233 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.32666028 ABIN2708234 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32666028 ABIN2064358 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32666028 ABIN2762215 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32666028 ABIN2113567 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
0.0012121415 ABIN365798 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012121415 ABIN3032929 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.05-500 pg/mL 2.05 pg/mL Log in to see
0.0012121415 ABIN509657 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012121415 ABIN624988 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 2
0.0012121415 ABIN626465 Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012121415 ABIN510464 Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012121415 ABIN1028755 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012121415 ABIN1446037 Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see
0.0012121415 ABIN1114291 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012121415 ABIN1112616 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced CSF2 ELISA Kit for ELISA

  • Human CSF2 ELISA Kit for Sandwich ELISA - ABIN624988 (2 Publications): Lu, Clauser, Tam, Fröse, Ye, Eaton, Reinhardt, Donnenberg, Bhargava, Carr, Weinberg: A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. in Nature cell biology 2014 (PubMed)

  • Human CSF2 ELISA Kit for Sandwich ELISA - ABIN414381 (1 Publications): Pollard, Rockstroh, Pantaleo, Asmuth, Peters, Lazzarin, Garcia, Ellefsen, Podzamczer, van Lunzen, Arastéh, Schürmann, Clotet, Hardy, Mitsuyasu, Moyle, Plettenberg, Fisher, Fätkenheuer, Fischl, Taiwo et al.: Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4x: a phase 2 randomised, double-blind, placebo-controlled trial. ... in The Lancet. Infectious diseases 2014 (PubMed)

Similar CSF2 ELISA Kits

Application / Reactivity Human
ELISA 43 ELISA Kits

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kits
Epitope AA 18-144
Reactivity Human
Kits with alternative reactivity to:
(47), (45), (34), (11), (9), (7), (5), (5), (4), (4), (3), (2)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CSF2 ELISA Kit

Target details Application Details Handling References for CSF2 Kit (ABIN1672729) Images
Purpose For quantitative detection of human GM-CSF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human GM-CSF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- human GM-CSF antibod - 1
  • Lyophilized recombinant human GM-CSF standar - 10ng/tubex2
  • Biotinylated anti- human GM-CSF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A18-E144

Target details

Product Details CSF2 ELISA Kit Application Details Handling References for CSF2 Kit (ABIN1672729) Images back to top
Antigen
Alternative Name CSF2 (CSF2 ELISA Kit Abstract)
Background Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69 % nucleotide homology and 54 % amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
Gene ID 1437
UniProt P04141
Pathways JAK-STAT Signaling

Application Details

Product Details CSF2 ELISA Kit Target details Handling References for CSF2 Kit (ABIN1672729) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human GM-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GM-CSF has been precoated onto 96-well plates. Standards(E.coli, A18-E144) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GM-CSF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human GM-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GM-CSF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 4.58, CV(%): 4.4
  • Sample 2: n=16, Mean(pg/ml): 485, Standard deviation: 15.52, CV(%): 3.2
  • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 27.95, CV(%): 4.5,
  • Sample 1: n=24, Mean(pg/ml): 107, Standard deviation: 6.53, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 463, Standard deviation: 29.63, CV(%): 6.4
  • Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 35.23, CV(%): 5.7
Restrictions For Research Use only

Handling

Product Details CSF2 ELISA Kit Target details Application Details References for CSF2 Kit (ABIN1672729) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CSF2 Kit (ABIN1672729)

Product Details CSF2 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Grabstein, Urdal, Tushinski, Mochizuki, Price, Cantrell, Gillis, Conlon: "Induction of macrophage tumoricidal activity by granulocyte-macrophage colony-stimulating factor." in: Science (New York, N.Y.), Vol. 232, Issue 4749, pp. 506-8, 1986

Huebner, Isobe, Croce, Golde, Kaufman, Gasson: "The human gene encoding GM-CSF is at 5q21-q32, the chromosome region deleted in the 5q- anomaly." in: Science (New York, N.Y.), Vol. 230, Issue 4731, pp. 1282-5, 1986

Images

Product Details CSF2 ELISA Kit Target details Application Details Handling References for CSF2 Kit (ABIN1672729) back to top
Supplier Images
ELISA image for CSF2 ELISA Kit (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) (ABIN1672729) Human GM-CSF PicoKine ELISA Kit standard curve