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anti-Human CSF2RB antibody for Flow Cytometry

Recommended CSF2RB Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) Antibodies
  • AI848964
  • AIC2B
  • Bc
  • CD131
  • cd131
  • CDw131
  • cdw131
  • CSF2RB
  • Csf2rb1
  • Csfgmrb
  • il-3rbc
  • Il3r
  • Il3rb
  • IL3RB
  • il3rb
  • Il3rb1
  • Il5rb
  • il5rb
  • IL5RB
  • SMDP5
Reactivity
Human
98
8
7
2
Host
Mouse
67
31
1
Clonality
Monoclonal
Conjugate
This CSF2RB antibody is un-conjugated
9
8
7
5
5
5
Application
Flow Cytometry (FACS)
69
49
18
16
9
7
6
2
1
1
1
Supplier
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Catalog No. ABIN487472
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30402437 ABIN2663003 FACS PE Mouse IgG1 kappa Log in to see 1C1 6
0.30402437 ABIN2664200 FACS Mouse IgG1 kappa Log in to see 1C1 5
0.30402437 ABIN1997020 FACS PE Mouse IgG1 AA 1-443 Log in to see 1
0.30402437 ABIN2677471 FACS Biotin Mouse IgG1 kappa Log in to see 1C1
0.0010469276 ABIN2855774 FACS WB Rabbit IgG Center Log in to see Polyclonal
0.0010469276 ABIN967466 FACS IP WB Mouse IgG1, kappa Log in to see 3D7 4
0.0010469276 ABIN2688952 FACS Biotin Mouse IgG1, kappa Log in to see 3D7 4
0.0010469276 ABIN205442 FACS IP WB Mouse IgG1 Log in to see 1C1
0.0010469276 ABIN474804 FACS PE Mouse IgG1, kappa Log in to see 1C1
0.0010469276 ABIN1822600 FACS IP WB Mouse IgG1, kappa Log in to see FIB504
0.0010469276 ABIN476001 FACS IP WB Mouse IgG1, kappa Log in to see 1C1
0.0010469276 ABIN2291940 FACS IP WB Mouse IgG1 kappa Log in to see 1C1
0.0010469276 ABIN278711 FACS PE Mouse IgG1 Log in to see 1C1
0.0010469276 ABIN1818244 FACS Mouse IgG1 Log in to see 1C1
0.0010469276 ABIN4264496 FACS FITC Mouse IgG1 kappa Log in to see 1C1
0.0010469276 ABIN4299727 FACS IP WB Mouse IgG1 kappa Log in to see 1C1
0.0010469276 ABIN2681831 FACS APC Mouse Log in to see
0.0010469276 ABIN2681828 FACS Mouse Log in to see

Top referenced anti-CSF2RB antibody for Flow Cytometry

  • Human Monoclonal CSF2RB Primary Antibody for FACS, IP - ABIN967466 (4 Publications): Woodcock, Zacharakis, Plaetinck, Bagley, Qiyu, Hercus, Tavernier, Lopez: Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF. in The EMBO journal 1994 (PubMed)

  • Human Monoclonal CSF2RB Primary Antibody for FACS - ABIN2688952 (4 Publications): Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: CD molecules 2006--human cell differentiation molecules. in Journal of immunological methods 2007 (PubMed)

  • Human Monoclonal CSF2RB Primary Antibody for FACS - ABIN2663003 (6 Publications): Lopez, Vadas, Woodcock, Milton, Lewis, Elliott, Gillis, Ireland, Olwell, Park: Interleukin-5, interleukin-3, and granulocyte-macrophage colony-stimulating factor cross-compete for binding to cell surface receptors on human eosinophils. in The Journal of biological chemistry 1992 (PubMed)

Similar anti-CSF2RB Antibodies

Application / Reactivity Human
Dot Blot (DB) 7 Antibodies
ELISA 49 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 19 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 69 Antibodies

General

Antigen Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) Antibodies
Reactivity Human
(98), (8), (7), (2)
Host Mouse
(67), (31), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2RB antibody is un-conjugated
(9), (8), (7), (5), (5), (5)
Application Flow Cytometry (FACS)
(69), (49), (18), (16), (9), (7), (6), (2), (1), (1), (1)
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Product Details anti-CSF2RB Antibody

Target Details CSF2RB Application Details Handling Images
Specificity This antibody reacts with Human CDw131 antigen (common beta subunit of IL-3, GMCSF and IL-5 receptors).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: IL3RB, IL5RB, GM-CSF-RB, Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5receptor common beta-chain
Purification Protein-A Sepharose Chromatography
Immunogen COS cells overexpressing GM-CSF receptor beta-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte
Clone 1C1
Isotype IgG1

Target Details CSF2RB

Product Details anti-CSF2RB Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD131 / CSF2RB (CSF2RB Antibody Abstract)
Background IL3R common beta chain (IL3RB)is a member of the Ig superfamily and a type I transmembrane glycoprotein. The common beta subunit associates with the specific alpha subunits of IL3 receptor, IL5 receptor and GMCSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response.Synonyms: Cytokine receptor common subunit beta, GM-CSF-RB, GM-CSF/IL-3/IL-5 receptor common beta-chain, IL3RB, IL5RB
Gene ID 1439
UniProt P32927
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CSF2RB Antibody Target Details CSF2RB Handling Images back to top
Application Notes Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Transfectant cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Flow Cytometric analysis for floating cellsProtocol 1We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500xg for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well, and incubate for 5 minutes at room temperature(20~25°C). 5) Add 30 µL of the CDw131 monoclonal antibody (10-20 µg/mL) diluted with the washingbuffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 7) Add 30 µL of secondary antibody (1: 40 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 8) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the CDw131 monoclonal antibody (50 µg/mL) diluted with the washingbuffer into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 50 ìL of the washing buffer. 7) Add 20 µL of secondary antibody (1: 10 FITC conjugated anti-mouse IgG diluted with thewashing buffer into each tube. Mix well and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all step described below. 1) Add 20 µL of the CDw131 monoclonal antibody (50 µg/mL) diluted with washing buffer[PBS containing 2% fetal calf serum (FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Add 2 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 50 µL of secondary antibody (1: 20 FITC conjugated anti-mouse IgG diluted with the
Restrictions For Research Use only

Handling

Product Details anti-CSF2RB Antibody Target Details CSF2RB Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CSF2RB Antibody Target Details CSF2RB Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CSF2RB antibody (Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage)) (ABIN487472) anti-Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) antibody