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anti-Human EPO antibody for Functional Studies

Recommended EPO Antibody (supplied by: Log in to see )

Antigen
Erythropoietin (EPO) Antibodies
  • EP
  • EPO
  • MVCD2
Reactivity
Human
280
52
31
5
2
1
1
Host
Rabbit
164
137
4
3
3
1
Clonality
Polyclonal
Conjugate
This EPO antibody is un-conjugated
25
19
14
8
6
6
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
214
205
19
16
11
11
10
7
7
6
4
4
3
3
3
2
2
1
1
Supplier
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Catalog No. ABIN112633
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008758766 ABIN609568 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008758766 ABIN198266 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008758766 ABIN2473500 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.0008758766 ABIN260347 Func IHC (p) IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008758766 ABIN640564 Func IHC (p) ELISA WB Rabbit IgG Log in to see

Top referenced anti-EPO antibody for Functional Studies

Similar anti-EPO Antibodies

Application / Reactivity Human
ELISA 192 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 6 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Neutralization (Neut) 1 Antibodies
Protein Assay (PrA) 2 Antibodies
Western Blotting (WB) 200 Antibodies

General

Antigen Erythropoietin (EPO) Antibodies
Reactivity Human
(280), (52), (31), (5), (2), (1), (1)
Host Rabbit
(164), (137), (4), (3), (3), (1)
Clonality
Polyclonal
Conjugate This EPO antibody is un-conjugated
(25), (19), (14), (8), (6), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(214), (205), (19), (16), (11), (11), (10), (7), (7), (6), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-EPO Antibody

Target Details EPO Application Details Handling References for anti-EPO Antibody (ABIN112633) Images
Specificity This antibody reacts with Erythropoietin, the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.
Purification Affinity Chromatography on Protein A
Immunogen CHO cell-derived, recombinant Human Erythropoietin.

Target Details EPO

Product Details anti-EPO Antibody Application Details Handling References for anti-EPO Antibody (ABIN112633) Images back to top
Antigen
Alternative Name Erythropoietin / EPO (EPO Antibody Abstract)
Background Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.Synonyms: Epoetin
Gene ID 2056
NCBI Accession NP_000790
UniProt P01588
Pathways JAK-STAT Signaling

Application Details

Product Details anti-EPO Antibody Target Details EPO Handling References for anti-EPO Antibody (ABIN112633) Images back to top
Application Notes ELISA: 0.5-1 μg/mL. Western Blot: 1-2 μg/mL (Reducing and Non-Reducing Conditions). Functional Assays: The ND50 was determined to be approximately 1-3 μg/mL, in thepresence of 0.3 units/mL of rhEpo, using a Human factor dependant cell line. Immunohistochemistry on Paraffin Sections: 15 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EPO Antibody Target Details EPO Application Details References for anti-EPO Antibody (ABIN112633) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2, 0.09 % Sodium Azide, 5 % Trehalose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-EPO Antibody (ABIN112633)

Product Details anti-EPO Antibody Target Details EPO Application Details Handling Images back to top
Background publications

Krystal: "A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice." in: Experimental hematology, Vol. 11, Issue 7, pp. 649-60, 1983

Kessler: "Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 115, Issue 6, pp. 1617-24, 1976